Chen D, Chen Z, Wang Z, Yang Y, Jiang Y, Hu C
Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Dec 20;41(12):1843-1849. doi: 10.12122/j.issn.1673-4254.2021.12.13.
To investigate the anti-tumor effect of BSA@CuS-PEG nanocomposites on tongue squamous cell carcinoma.
Transmission electron microscopy, dynamic light scattering, Zeta potential and ultraviolet absorption spectroscopy were used to characterize the synthesized BSA@CuS-PEG nanocomposite, whose photothermal properties was assessed with near infrared Ⅱ region excitation light (1064 nm). The cytotoxicity of the nanocomposite in Cal27 and SCC9 cells was evaluated using CCK-8 assay, and its effect on cell cycle distribution was analyzed using flow cytometry. The antitumor effect of BSA@CuS-PEG was investigated in a Balb/c mouse model bearing subcutaneous Cal27 tumor xenograft.
The synthesized BSA@CuS-PEG nanocomposite showed a temperature variation (ΔT) of about 30 ℃ under near-infrared (NIR) irradiation (0.5 W/cm2), suggesting its excellent photothermal sensitivity. CCK-8 assay showed that BSA@CuS-PEG had no significant toxicity to tumor cells, but upon NIR irradiation, the nanocomposite produced a significant stronger inhibitory effect on Cal27 and SCC9 cells than free nanocomposites ( < 0.001). Cell cycle analysis showed that compared with free nanocomposites, BSA@CuS-PEG plus NIR irradiation caused more obvious cell cycle arrest at G2/M phase in tongue cancer cells ( < 0.001). In the tumor-bearing mice, BSA@CuS-PEG combined with NIR irradiation produced a significant anti-tumor effect as compared with saline treatment plus NIR irradiation ( < 0.001).
The BSA@CuS-PEG nanocomposite shows prominent photothermal properties and good anti-tumor effects both and , and thus provides a promising method for non-invasive early diagnosis and non-surgical treatment of primary tongue cancer.
研究牛血清白蛋白@硫化铜-聚乙二醇（BSA@CuS-PEG）纳米复合材料对舌鳞状细胞癌的抗肿瘤作用。
采用透射电子显微镜、动态光散射、Zeta电位和紫外吸收光谱对合成的BSA@CuS-PEG纳米复合材料进行表征，并用近红外Ⅱ区激发光（1064nm）评估其光热性能。采用CCK-8法评价纳米复合材料对Cal27和SCC9细胞的细胞毒性，并用流式细胞术分析其对细胞周期分布的影响。在携带皮下Cal27肿瘤异种移植的Balb/c小鼠模型中研究BSA@CuS-PEG的抗肿瘤作用。
合成的BSA@CuS-PEG纳米复合材料在近红外（NIR）照射（0.5W/cm2）下温度变化（ΔT）约为30℃，表明其具有优异的光热敏感性。CCK-8法显示，BSA@CuS-PEG对肿瘤细胞无明显毒性，但在NIR照射下，纳米复合材料对Cal27和SCC9细胞的抑制作用明显强于游离纳米复合材料（P<0.001）。细胞周期分析表明，与游离纳米复合材料相比，BSA@CuS-PEG加NIR照射使舌癌细胞在G2/M期的细胞周期阻滞更明显（P<0.001）。在荷瘤小鼠中，与生理盐水处理加NIR照射相比，BSA@CuS-PEG联合NIR照射产生了显著的抗肿瘤作用（P<0.001）。
BSA@CuS-PEG纳米复合材料在体外和体内均表现出突出的光热性能和良好的抗肿瘤作用，为原发性舌癌的非侵入性早期诊断和非手术治疗提供了一种有前景的方法。
