Dong Mingjun, Xie Yangyang, Xu Yidong
Department of Anorectal Surgery, The No. 2 Hospital of Ningbo, Ningbo, Zhejiang 315010, P.R. China.
Oncol Lett. 2019 Mar;17(3):3241-3246. doi: 10.3892/ol.2019.10001. Epub 2019 Feb 1.
Previous studies have demonstrated that microRNA-7-5p (miR-7-5p) functions as a tumor suppressor in certain types of human cancer. However, the role of miR-7-5p in colorectal cancer (CRC) remains to be investigated. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the present study demonstrated that miR-7-5p was downregulated in CRC tissues and cell lines. In addition, low miR-7-5p expression is able to predict a poor 5-year overall survival rate for patients with CRC. studies revealed that miR-7-5p overexpression inhibits cell proliferation and migration. Furthermore, Krüppel-like factor 4 (KLF4), an oncogene in CRC, was validated as a direct target of miR-7-5p. KLF4 expression was negatively correlated with miR-7-5p expression in CRC tissues. Notably, KLF4 overexpression rescued the suppressive effects of miR-7-5p on CRC cell proliferation and migration. In summary, the results of this study demonstrated that miR-7-5p inhibits CRC proliferation and migration by targeting KLF4, which suggests that miR-7-5p is a potential molecular target for the treatment of human CRC.
先前的研究表明，微小RNA-7-5p（miR-7-5p）在某些类型的人类癌症中发挥肿瘤抑制作用。然而，miR-7-5p在结直肠癌（CRC）中的作用仍有待研究。本研究通过逆转录定量聚合酶链反应（RT-qPCR）证明，miR-7-5p在CRC组织和细胞系中表达下调。此外，miR-7-5p低表达能够预测CRC患者较差的5年总生存率。研究表明，miR-7-5p过表达抑制细胞增殖和迁移。此外，Krüppel样因子4（KLF4）是CRC中的一种癌基因，被证实为miR-7-5p的直接靶点。在CRC组织中，KLF4表达与miR-7-5p表达呈负相关。值得注意的是，KLF4过表达挽救了miR-7-5p对CRC细胞增殖和迁移的抑制作用。总之，本研究结果表明，miR-7-5p通过靶向KLF4抑制CRC增殖和迁移，这表明miR-7-5p是治疗人类CRC的潜在分子靶点。
