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[用于诊断铜绿假单胞菌感染的乳胶凝集法的开发]

[Development of a latex agglutination method for the diagnosis of Pseudomonas aeruginosa infection].

作者信息

Aleksandrova I A, Antsiferova N G, Aleksandrov A D, Moroz A F

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1986 Apr(4):10-4.

PMID:3087117
Abstract

The present investigation has revealed the possibility of using different kinds of monodispersed polystyrene latex, produced in the USSR, as carriers in the process of the preparation of antibody diagnosticums intended for the detection of water-soluble slime antigens of Pseudomonas aeruginosa strains belonging to the most widespread serological types. The optimum conditions for the preparation of latex reagents and for making the latex-agglutination test have been experimentally established. The new diagnosticums+ have been shown to be highly species- and type-specific, which permits making judgment on the presence or absence of slime antigens of P. aeruginosa strains belonging to definite serovars in the clinical material under study. The preparations thus obtained have been found to retain their sensitivity for 16 months (the term of observation).

摘要

目前的研究揭示了使用苏联生产的不同种类单分散聚苯乙烯乳胶作为载体的可能性,用于制备旨在检测属于最广泛血清型的铜绿假单胞菌菌株水溶性黏液抗原的抗体诊断制剂。通过实验确定了制备乳胶试剂和进行乳胶凝集试验的最佳条件。新的诊断制剂已显示出高度的种属和型特异性,这使得能够判断所研究临床材料中是否存在属于特定血清型的铜绿假单胞菌菌株的黏液抗原。已发现由此获得的制剂在16个月(观察期)内保持其敏感性。

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