Lages Lucas C, Lopez Johanna, Lopez-Medrano Ana Maria, Atlas Steven E, Martinez Ana H, Woolger Judi M, Tiozzo Eduard, Konefal Janet, Mendez Armando J, Simoes Herbert G, Lewis John E
Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, United States.
Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, United States.
J Clin Transl Res. 2017 Jan 3;2(4):135-143. eCollection 2017 Jan 4.
Nutritional approaches that ameliorate cellular senescence may have the potential to counteract the effects of chronic disease. This study will investigate the effect of the Healthycell dietary supplement on markers of inflammation, oxidative stress, and DNA damage. Thirty adults between the ages of 18 and 55 were enrolled and randomly assigned to one of the two study conditions (n = 15 Healthycell and n = 15 placebo). Subjects participated in a four-week intervention and were assessed at baseline, four weeks, and six weeks (after a two-week washout period). Pro-inflammatory cytokine interleukin (IL)-1α (t = 2.033; mean difference = -3.97 pg/ml; SE = 2.0; 95% CI: -8.0, -0.3; Cohen's d = 0.77; p = 0.05) decreased, while soluble cytokine receptors sTNFR-I (t = 2.057; mean difference = 52.39 pg/mL; SE = 18.5; 95% CI: 5.2, 99.6; Cohen's d = 0.53; = 0.03) and sTNFR-II (t = 1.739; mean difference = 208.71 pg/ml; SE = 72.0; 95% CI: 24.4, 393.0; Cohen's d = 0.61; = 0.02) increased in the treatment group versus control. C-reactive protein also rose in the Healthycell group during the trial (t = 2.568; mean difference = 1.41 mg/dL; SE = 0.4; 95% CI: 0.3, 2.5; Cohen's d = 0.66; p < 0.01), without accompanying increases in IL-6 and TNF-α. Additionally, cortisol levels decreased in the Healthycell group (t = 0.575; mean difference = -0.31 ug/dL; SE=0.1; 95% CI: -0.6, -0.03; Cohen's d = 0.88; = 0.03). When groups were split by age (< 35 years vs. ≥ 35 years), 8-hydroxydeoxyguanosine, a marker of DNA damage, decreased in the older Healthycell group compared to placebo (t = 1.782; mean difference = -7.09 ng/mL; SE = 3.0; 95% CI: -13.3, -0.9; Cohen's d = 0.63; = 0.03). Significant changes were also found for sTNFR-I, sTNFR-II, and IL-5 in the older group. All results were obtained from t tests by post-hoc analysis. Our findings show an improved inflammatory profile and decreased DNA damage. Additionally, the efficacy of Healthycell was primarily in older adults, where the processes that cause or are associated with cell senescence are more predominant. Healthycell may help to counteract the inflammatory effects of aging that lead to both cell senescence and the multitude of age-related chronic diseases.
改善细胞衰老的营养方法可能具有抵消慢性疾病影响的潜力。本研究将调查健康细胞膳食补充剂对炎症、氧化应激和DNA损伤标志物的影响。招募了30名年龄在18至55岁之间的成年人，并将他们随机分配到两种研究条件之一（n = 15为健康细胞组，n = 15为安慰剂组）。受试者参与了为期四周的干预，并在基线、四周和六周（经过两周的洗脱期后）进行评估。治疗组与对照组相比，促炎细胞因子白细胞介素（IL）-1α（t = 2.033；平均差异 = -3.97 pg/ml；标准误 = 2.0；95%置信区间：-8.0，-0.3；科恩d值 = 0.77；p = 0.05）降低，而可溶性细胞因子受体sTNFR-I（t = 2.057；平均差异 = 52.39 pg/mL；标准误 = 18.5；95%置信区间：5.2，99.6；科恩d值 = 0.53；p = 0.03）和sTNFR-II（t = 1.739；平均差异 = 208.71 pg/ml；标准误 = 72.0；95%置信区间：24.4，393.0；科恩d值 = 0.61；p = 0.02）升高。在试验期间，健康细胞组中的C反应蛋白也有所升高（t = 2.568；平均差异 = 1.41 mg/dL；标准误 = 0.4；95%置信区间：0.3，2.5；科恩d值 = 0.66；p < 0.01），而IL-6和TNF-α没有随之升高。此外，健康细胞组中的皮质醇水平降低（t = 0.575；平均差异 = -0.31 ug/dL；标准误 = 0.1；95%置信区间：-0.6，-0.03；科恩d值 = 0.88；p = 0.03）。当按年龄分组（< 35岁与≥ 35岁）时，与安慰剂相比，老年健康细胞组中DNA损伤标志物8-羟基脱氧鸟苷降低（t = 1.782；平均差异 = -7.09 ng/mL；标准误 = 3.0；95%置信区间：-13.3，-0.9；科恩d值 = 0.63；p = 0.03）。在老年组中，sTNFR-I、sTNFR-II和IL-5也有显著变化。所有结果均通过事后分析的t检验获得。我们的研究结果显示炎症特征得到改善，DNA损伤减少。此外，健康细胞的功效主要体现在老年人中，在老年人中导致或与细胞衰老相关的过程更为突出。健康细胞可能有助于抵消导致细胞衰老和多种与年龄相关的慢性疾病的衰老炎症效应。
