Bossi Renata de Lima, Valadares Jéssica Bruna Fernandes, Puerto Helen Lima Del, Rocha Maria Gabrielle Lima, Braga Letícia Conceição, Sampaio Marcos Aurelio Coelho, França Patrícia Pinho, Alvarenga Débora Moreira, Geber Selmo
Centro de Medicina Reprodutiva- Belo Horizonte/MG- Brazil.
Departamento de Patologia Geral do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais- Brazil.
JBRA Assist Reprod. 2019 Aug 22;23(3):205-209. doi: 10.5935/1518-0557.20190009.
The aim of our study was to identify the prevalence of HPV in the semen of men submitted to ART treatment and look into the possible impacts of the virus on sperm parameters.
Thirty-five patients treated for infertility from March to August 2016 were invited to join the study. Samples with a minimum concentration of 40x106 spermatozoa per milliliter were included in the study. After the evaluation of semen parameters, DNA extraction and PCR were performed to verify the presence of HPV by electrophoresis in 8% polyacrylamide gel.
Patient age ranged from 27 to 68 years (mean 39.2 years). Semen analysis showed a mean volume of 2.5mL; mean concentration of 58.9x10; and mean motility of 51.8%. HPV DNA was identified in seven semen samples from 25 patients (28%). Ten samples with DNA concentrations below 10ng/µL were excluded from the study due to poor amplification quality. There was no statistical difference in sperm concentration when HPV-negative and HPV-positive samples were compared (65.9x10 vs. 62.3x10; p=0.70). However, sperm motility was significantly higher in HPV-positive semen (65% vs. 46.6%; p=0.02).
HPV prevalence was 28% in the semen of patients submitted to ART treatment. HPV-positive samples had statistically increased motility compared to negative samples (65% vs. 46.6%; p=0.02).
我们研究的目的是确定接受辅助生殖技术（ART）治疗的男性精液中人类乳头瘤病毒（HPV）的流行率，并探究该病毒对精子参数可能产生的影响。
邀请了2016年3月至8月间接受不育治疗的35名患者参与研究。研究纳入每毫升精子浓度至少为40×10⁶的样本。在评估精液参数后，进行DNA提取和聚合酶链反应（PCR），通过8%聚丙烯酰胺凝胶电泳来验证HPV的存在。
患者年龄在27至68岁之间（平均39.2岁）。精液分析显示平均体积为2.5mL；平均浓度为58.9×10；平均活力为51.8%。在25名患者的7份精液样本中鉴定出HPV DNA（28%）。由于扩增质量差，10份DNA浓度低于10ng/µL的样本被排除在研究之外。比较HPV阴性和阳性样本时，精子浓度没有统计学差异（65.9×10对62.3×10；p = 0.70）。然而，HPV阳性精液中的精子活力显著更高（65%对46.6%；p = 0.02）。
接受ART治疗的患者精液中HPV流行率为28%。与阴性样本相比，HPV阳性样本的活力在统计学上有所增加（65%对46.6%；p = 0.02）。
