State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China; Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Biosens Bioelectron. 2019 May 1;132:224-229. doi: 10.1016/j.bios.2019.02.053. Epub 2019 Mar 4.
Rapid detection of Pseudomonas aeruginosa (P. aeruginosa) is of great importance for accurate diagnosis and treatment of infected patients. In this study, a novel method was developed for the selective detection of P. aeruginosa by combing the sandwich type complex of magnetic bead/aptamer/polyadenylated-DNA with the sensitive detection platform of gold (Au) interdigital electrode connected to a multichannel series piezoelectric quartz crystal (Au IDE-MSPQC) system. Here, the magnetic bead (MB) was used as carrier for immobilization of the aptamer of P. aeruginosa. Polyadenylated DNA was bound to the aptamer through complementary strand pairing. When the P. aeruginosa was present in the sample solution, the polyadenylated DNA was replaced by the P. aeruginosa because of the specific interaction between P. aeruginosa and its aptamer. The released polyadenylated DNA strand in the detected solution could adsorb onto the surface of Au IDE by virtue of the strong interaction between adenine (A) and Au IDE, and result in sensitive frequency shift response of the MSPQC sensor. The limits of detection (LOD) of the method were as low as 9 CFU/mL in buffer and 52 CFU/mL in simulated blood sample. The proposed method was successfully applied to the selective detection of P. aeruginosa in blood samples. The constructed sensor is expected to find application for the rapid detection of P. aeruginosa in environment, food and clinical diagnosis.
快速检测铜绿假单胞菌（P. aeruginosa）对于感染患者的准确诊断和治疗非常重要。在本研究中，通过将磁珠/适体/聚腺苷酸化 DNA 的三明治型复合物与连接到多通道串联式压电石英晶体（Au IDE-MSPQC）系统的金（Au）叉指电极的灵敏检测平台相结合，开发了一种用于选择性检测铜绿假单胞菌的新方法。在这里，磁珠（MB）用作铜绿假单胞菌适体的固定化载体。聚腺苷酸化 DNA 通过互补链配对结合到适体上。当样品溶液中存在铜绿假单胞菌时，由于铜绿假单胞菌与其适体之间的特异性相互作用，聚腺苷酸化 DNA 被铜绿假单胞菌取代。在检测溶液中释放的聚腺苷酸化 DNA 链可以通过腺嘌呤（A）与 Au IDE 之间的强相互作用吸附到 Au IDE 表面，从而导致 MSPQC 传感器的敏感频率位移响应。该方法在缓冲液中的检测限（LOD）低至 9 CFU/mL，在模拟血液样本中的检测限低至 52 CFU/mL。该方法成功应用于血液样本中铜绿假单胞菌的选择性检测。所构建的传感器有望在环境、食品和临床诊断中用于快速检测铜绿假单胞菌。
Zotero 插件