Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Agricultural Technology Promotion Station of Taizhou, Taizhou, 318000, China.
BMC Plant Biol. 2019 Mar 19;19(1):106. doi: 10.1186/s12870-019-1717-y.
MADS-box genes play important roles in vegetative growth and reproductive development and are essential for the correct development of plants (particularly inflorescences, flowers, and fruits). However, this gene family has not been identified nor their functions analyzed in Brassica oleracea.
In this study, we performed a whole-genome survey of the complete set of MADS-box genes in B. oleracea. In total, 91 MADS-box transcription factors (TFs) were identified and categorized as type I (Mα, Mβ, Mγ) and type II (MIKC, MIKC*) groups according to the phylogeny and gene structure analysis. Among these genes, 59 were randomly distributed on 9 chromosomes, while the other 23 were assigned to 19 scaffolds and 9 genes from NCBI had no location information. Both RNA-sequencing and quantitative real-time-PCR analysis suggested that MIKC genes had more active and complex expression patterns than M type genes and most type II genes showed high flowering-related expression profiles. Additional quantitative real-time-PCR analysis of pedicel and four flower whorls revealed that the structure of the B.oleracea MIKC genes was conserved, but their homologues showed variable expression patterns compared to those in Arabidopsis thaliana.
This paper gives a detailed overview of the BolMADS genes and their expression patterns. The results obtained in this study provide useful information for understanding the molecular regulation of flower development and further functional characterization of MADS-box genes in B. oleracea.
MADS 盒基因在营养生长和生殖发育中发挥重要作用，是植物正确发育（特别是花序、花和果实）所必需的。然而，在甘蓝型油菜中，尚未鉴定出该基因家族，也未分析其功能。
本研究对甘蓝型油菜中完整的 MADS 盒基因进行了全基因组调查。共鉴定出 91 个 MADS 盒转录因子（TF），根据系统发育和基因结构分析分为 I 型（Mα、Mβ、Mγ）和 II 型（MIKC、MIKC*）。其中 59 个基因随机分布在 9 条染色体上，而另外 23 个基因被分配到 19 个支架上，9 个来自 NCBI 的基因没有位置信息。RNA-seq 和定量实时 PCR 分析均表明，MIKC 基因的表达模式比 M 型基因更活跃、更复杂，大多数 II 型基因具有较高的开花相关表达谱。对花梗和四轮花的进一步定量实时 PCR 分析表明，甘蓝型油菜 MIKC 基因的结构保守，但与拟南芥相比，其同源基因表现出不同的表达模式。
本文详细概述了 BolMADS 基因及其表达模式。本研究的结果为了解花发育的分子调控以及进一步对甘蓝型油菜 MADS 盒基因的功能特征提供了有用信息。
