Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, School of Dentistry , University of California, Los Angeles , Los Angeles , California 90095-1668 , United States.
California NanoSystems Institute , University of California, Los Angeles , 570 Westwood Plaza , Los Angeles , California 90095-7227 , United States.
ACS Nano. 2019 Apr 23;13(4):3830-3838. doi: 10.1021/acsnano.8b09623. Epub 2019 Mar 21.
Periodontitis is a common chronic inflammatory disease that affects tooth-supporting tissues. We engineer a multifunctional periodontal membrane for the guided tissue regeneration of lost periodontal tissues. The major drawback of current periodontal membranes is the lack of tissue regeneration properties. Here, a series of nanofibrous membranes based on poly(ε-caprolactone) with tunable biochemical and biophysical properties were developed for periodontal tissue regeneration. The engineered membranes were surface coated using biomimetic polydopamine to promote the adhesion of therapeutic proteins and cells. We demonstrate successful cellular localization on the surface of the engineered membrane by morphological patterning. Polydopamine accelerates osteogenic differentiation of dental-derived stem cells by promoting hydroxyapatite mineralization. Such multiscale designs can mimic the complex extracellular environment of periodontal tissue and serve as functional tissue constructs for periodontal regeneration. In a periodontal defect model in rats, our engineered periodontal membrane successfully promoted the regeneration of periodontal tissue and bone repair. Altogether, our data demonstrate that our biomimetic membranes have potential as protein/cell delivery platforms for periodontal tissue engineering.
牙周炎是一种常见的慢性炎症性疾病，影响牙齿支持组织。我们为引导组织再生术设计了一种多功能牙周膜，用于再生丧失的牙周组织。目前牙周膜的主要缺点是缺乏组织再生特性。在这里，我们开发了一系列基于聚己内酯的具有可调生化和生物物理特性的纳米纤维膜，用于牙周组织再生。通过仿生聚多巴胺对工程膜进行表面涂层处理，以促进治疗性蛋白和细胞的黏附。我们通过形态图案化证明了治疗性蛋白和细胞在工程膜表面的成功细胞定位。聚多巴胺通过促进羟基磷灰石矿化来加速牙源性干细胞的成骨分化。这种多尺度设计可以模拟牙周组织的复杂细胞外环境，并作为牙周再生的功能性组织构建体。在大鼠牙周缺损模型中，我们设计的牙周膜成功促进了牙周组织的再生和骨修复。总之，我们的数据表明，我们的仿生膜具有作为牙周组织工程中蛋白/细胞递药平台的潜力。
