State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China.
Mol Nutr Food Res. 2019 Jun;63(12):e1801008. doi: 10.1002/mnfr.201801008. Epub 2019 Apr 11.
This study is aimed to investigate the kidney protective effects of glucomannans from Dendrobium officinale stem, konjac, and Aloe vera leaves on type 2 diabetic rats and explore its potential mechanisms.
Pathological and metabolomics analysis methods are adopted in this study. Compared with the model group, lower levels of fasting blood glucose, serum insulin, and glycated serum protein are observed in glucomannan-treated groups. Concentrations of serum low-density lipoprotein cholesterol, total cholesterol, triacylglycerols, and nonesterified fatty acid are significantly decreased by glucomannan treatment. Furthermore, glucomannan treatment significantly decreased the levels of uric acid, creatinine, urea in serum, and glycosuria, ketone body, and protein in the urine. Histopathological analysis showed that glucomannan treatment normalized the architecture of glomerulus. Metabolomic analysis indicated glucomannan treatment could improve urea cycle, metabolism of lipid, glucose, and amino acids on diabetes. In particular, the konjac glucomannan treatment is more effective in lipid and glucose regulation. Glucomannan from D. officinale is more effective in balancing the urea cycle and amino acid metabolism.
The disturbance of lipid, glucose, and amino acid metabolism is closely associated with the advancement of diabetic kidney disease, and glucomannan treatment could be efficient in the management of diabetic kidney disease.
本研究旨在探讨铁皮石斛茎、魔芋和库拉索芦荟叶中的葡甘露聚糖对 2 型糖尿病大鼠的肾脏保护作用，并探讨其潜在机制。
本研究采用病理和代谢组学分析方法。与模型组相比，葡甘露聚糖治疗组空腹血糖、血清胰岛素和糖化血清蛋白水平较低。葡甘露聚糖治疗可显著降低血清低密度脂蛋白胆固醇、总胆固醇、三酰甘油和非酯化脂肪酸浓度。此外，葡甘露聚糖治疗可显著降低血清尿酸、肌酐、尿素水平，以及尿中的尿糖、酮体和蛋白质。组织病理学分析表明，葡甘露聚糖治疗可使肾小球结构正常化。代谢组学分析表明，葡甘露聚糖治疗可改善糖尿病患者的尿素循环、脂质代谢、葡萄糖代谢和氨基酸代谢。特别是魔芋葡甘露聚糖在调节脂质和葡萄糖方面更有效。铁皮石斛葡甘露聚糖在平衡尿素循环和氨基酸代谢方面更有效。
脂质、葡萄糖和氨基酸代谢的紊乱与糖尿病肾病的进展密切相关，葡甘露聚糖治疗可有效治疗糖尿病肾病。
