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基于氧敏膜的比率型荧光生物传感器用于葡萄糖和乳酸检测。

Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane.

机构信息

School of Chemical Engineering, Chonnam National University, Yong-Bong Ro 77, Gwangju 61186, Korea.

出版信息

Biosensors (Basel). 2021 Jun 25;11(7):208. doi: 10.3390/bios11070208.

DOI:10.3390/bios11070208
PMID:34202015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301843/
Abstract

In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1-2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1-0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

摘要

在这项研究中，使用固定有葡萄糖氧化酶 (GOD) 或乳酸氧化酶 (LOX) 的比率荧光氧敏膜，开发了比率荧光葡萄糖和乳酸生物传感器。在这里，比率荧光氧敏膜是由两种发射波长的铂中四 (五氟苯基) 卟啉 (PtP) 掺杂在聚苯乙烯颗粒中和香豆素 6 (C6) 捕获在硅颗粒中制成的。传感膜的工作机制基于 (i) 氧分子对 PtP 染料的荧光猝灭效应，以及 (ii) 在 GOD 或 LOX 的催化下葡萄糖或乳酸氧化反应中氧水平的消耗。比率荧光葡萄糖传感膜对 0.1-2 mM 范围内的葡萄糖表现出高灵敏度，检测限 (LOD) 为 0.031 mM，而比率荧光乳酸传感膜则显示出 0.1-0.8 mM 的线性检测范围，检测限 (LOD) 为 0.06 mM。这些传感膜还表现出良好的选择性、快速的可逆性和长期使用的稳定性。它们被应用于检测人工人血清中的葡萄糖和乳酸，并提供了可靠的测量结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/cdc650045376/biosensors-11-00208-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/447a77845662/biosensors-11-00208-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/d883d74aec1f/biosensors-11-00208-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/aa88aa491883/biosensors-11-00208-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/acd32d8fbd49/biosensors-11-00208-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/0bf53e2c4db4/biosensors-11-00208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/84c5eb9b0a8b/biosensors-11-00208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/8ffb41b131cd/biosensors-11-00208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/c7721ce2b21f/biosensors-11-00208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/5e832f46597f/biosensors-11-00208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/2bd99f5b76cc/biosensors-11-00208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/532bbaf059f1/biosensors-11-00208-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/bad982251c6a/biosensors-11-00208-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/503a2b7d3e7e/biosensors-11-00208-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/cdc650045376/biosensors-11-00208-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/447a77845662/biosensors-11-00208-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/d883d74aec1f/biosensors-11-00208-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/aa88aa491883/biosensors-11-00208-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/acd32d8fbd49/biosensors-11-00208-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/0bf53e2c4db4/biosensors-11-00208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/84c5eb9b0a8b/biosensors-11-00208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/8ffb41b131cd/biosensors-11-00208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/c7721ce2b21f/biosensors-11-00208-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/5e832f46597f/biosensors-11-00208-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/2bd99f5b76cc/biosensors-11-00208-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/532bbaf059f1/biosensors-11-00208-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/bad982251c6a/biosensors-11-00208-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/503a2b7d3e7e/biosensors-11-00208-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba22/8301843/cdc650045376/biosensors-11-00208-g012.jpg

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