Pol Laura, Eckstein Chris, Acosta Laura K, Xifré-Pérez Elisabet, Ferré-Borrull Josep, Marsal Lluis F
Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, ETSE, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain.
Nanomaterials (Basel). 2019 Mar 23;9(3):478. doi: 10.3390/nano9030478.
The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.
纳米材料表面的化学修饰或功能化是实现具有最佳灵敏度和选择性生物传感器的关键步骤。生物传感器的表面修饰通常包括几个需要优化的修饰步骤。在此类修饰步骤中对所有发生的反应进行实时监测可能是优化的一个非常有用的工具。在这项工作中，我们提出用链霉亲和素 - 生物素复合物功能化的纳米多孔阳极氧化铝（NAA）作为无标记生物传感器的平台。使用反射干涉光谱法（RIfS），以生物素化凝血酶作为分子模型，实时监测链霉亲和素 - 生物素复合物的形成。该研究比较了不同NAA孔径的性能以实现最高响应。此外，估计了能够有效检测生物素化凝血酶附着的最佳链霉亲和素浓度。最后，评估了NAA - RIfS系统定量生物素化凝血酶浓度的能力。本研究提供了一种优化的表征方法，用于监测在NAA孔内生物素化分子附着过程中发生的化学反应。
