College of Biological, Chemical Sciences and Engineering , Jiaxing University , Jiaxing , Zhejiang - 314001 , People's Republic of China.
Department of Material Engineering , KU Leuven , Kasteelpark Arenberg 44, P.O. Box 2450 , B-3001 Heverlee , Belgium.
Anal Chem. 2019 May 7;91(9):5824-5833. doi: 10.1021/acs.analchem.9b00153. Epub 2019 Apr 9.
In this work, we report a new biosensing platform for hepatitis B virus (HBV) DNA genosensing using cobalt oxide (CoO) nanostructures. The tunable morphologies of CoO nanostructures such as porous nanocubes (PNCs), nanooctahedra (NOHs), and nanosticks (NSKs) are synthesized, and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns, nitrogen adsorption/desorption isotherms (BET), and electrochemical impedance spectral (EIS) methods. The HBV probe DNA (ssDNA) is immobilized on the CoO nanostructures through coordinate bond formation between nucleic acid of ssDNA and Co metal, which results in highly stable nanostructured biosensing platform. To the best of our knowledge, first time the target cDNA of HBV is detected using ssDNA/CoOPNCs/GCE electrode by EIS method with a limit of detection (LOD) of 0.38 pM (signal-to-noise ratio (S/N) = 3). Moreover, the ssDNA/CoOPNCs/GCE has shown excellent specificity to HBV target cDNA, compared with noncomplementary DNA, and 1- and 3-mismatch DNAs. Finally, we explore ssDNA/CoOPNCs/GCE as potential electrode to test HBV DNA in blood serum and urine samples for practical applications.
在这项工作中，我们报告了一种使用氧化钴（CoO）纳米结构进行乙型肝炎病毒（HBV）DNA 基因传感的新生物传感平台。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线衍射（XRD）图谱、氮气吸附/解吸等温线（BET）和电化学阻抗谱（EIS）方法，合成了 CoO 纳米结构的可调形态，如多孔纳米立方体（PNC）、纳米八面体（NOH）和纳米棒（NSK）。HBV 探针 DNA（ssDNA）通过 ssDNA 与 Co 金属之间的配位键形成固定在 CoO 纳米结构上，从而形成高度稳定的纳米结构生物传感平台。据我们所知，这是首次通过电化学阻抗谱（EIS）方法使用 ssDNA/CoOPNCs/GCE 电极检测 HBV 的目标 cDNA，检测限（LOD）为 0.38 pM（信噪比（S/N）=3）。此外，与非互补 DNA 和 1 个和 3 个错配 DNA 相比，ssDNA/CoOPNCs/GCE 对 HBV 靶 cDNA 表现出优异的特异性。最后，我们探索了 ssDNA/CoOPNCs/GCE 作为潜在电极，用于测试血清和尿液样本中的 HBV DNA，以进行实际应用。
