Shapira S K, Finnerty V G
J Mol Evol. 1986;23(2):159-67. doi: 10.1007/BF02099910.
Gene duplications must play an important role in the evolutionary development of living organisms. Presented here is a general scheme that uses complementary alleles to isolate gene duplications in diploid organisms. The technique was used in Drosophila melanogaster to assess the rate of spontaneous gene duplication at two loci, maroon-like and rosy. The results indicate that the rate of duplication of the maroon-like locus is on the order of 2.7 X 10(-6); that the rate of duplication of the rosy locus is approximately 1.7 X 10(-4); and that duplication occurs in males, suggesting that there may actually be two modes of gene duplication in Drosophila melanogaster.
基因复制必定在生物体的进化发展中发挥着重要作用。本文提出了一种利用互补等位基因来分离二倍体生物中基因复制的通用方案。该技术被用于黑腹果蝇，以评估两个基因座（类栗色和玫瑰色）的自发基因复制率。结果表明，类栗色基因座的复制率约为2.7×10⁻⁶；玫瑰色基因座的复制率约为1.7×10⁻⁴；并且复制发生在雄性中，这表明黑腹果蝇中可能实际上存在两种基因复制模式。
