Khajoei Sholeh, Hassaninevisi Maryam, Kianmehr Nahid, Seif Farhad, Khoshmirsafa Majid, Shekarabi Mehdi, Samei Azam, Haghighi Anousheh
Department of Rheumatology, Iran University of Medical Sciences, Tehran, Iran.
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2019 Apr;46(2):2505-2512. doi: 10.1007/s11033-019-04682-1. Epub 2019 Mar 27.
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which numerous cells and mediators affect inflammatory conditions and disease severity. To compare the serum levels of adiponectin, vitamin D, copper, and zinc in patients with RA and to investigate the relationship between these parameters and RA severity. Ninety patients with RA and 30 healthy controls participated in this cross-sectional case-control study between November 2016 and April 2017; according to the ACR/EULAR criteria for RA. Serum levels of adiponectin were determined by ELISA; copper and zinc by colorimetric spectrophotometry; and vitamin D by HPLC. Kruskal-Wallis and Spearman tests were performed using SPSS software and data were depicted by GraphPad Prism software. Compared with healthy controls, the serum level of adiponectin was significantly increased, whereas vitamin D was significantly decreased in patients with RA. Adiponectin and vitamin D levels were inversely correlated in RA subgroups (P < 0.001, r = - 0.410). Adiponectin and vitamin D correlated with RA severity. Furthermore, no significant difference was found in copper and zinc levels between RA groups and controls. The definitive roles of adiponectin, vitamin D, copper, and zinc are not completely determined in RA development. Based on disease activity, these parameters can modulate inflammatory conditions, thus they have the potential to be used as promising therapeutic biomarkers to follow up the severity of disease, as well as the progression and treatment success in patients with RA.
类风湿关节炎（RA）是一种慢性炎症性疾病，其中众多细胞和介质会影响炎症状态和疾病严重程度。比较类风湿关节炎患者血清中脂联素、维生素D、铜和锌的水平，并研究这些参数与类风湿关节炎严重程度之间的关系。2016年11月至2017年4月期间，90例类风湿关节炎患者和30名健康对照参与了这项横断面病例对照研究；根据美国风湿病学会/欧洲抗风湿病联盟（ACR/EULAR）的类风湿关节炎标准。采用酶联免疫吸附测定法（ELISA）测定血清脂联素水平；用比色分光光度法测定铜和锌；用高效液相色谱法（HPLC）测定维生素D。使用SPSS软件进行Kruskal-Wallis和Spearman检验，并用GraphPad Prism软件呈现数据。与健康对照相比，类风湿关节炎患者血清脂联素水平显著升高，而维生素D水平显著降低。在类风湿关节炎亚组中，脂联素和维生素D水平呈负相关（P < 0.001，r = -0.410）。脂联素和维生素D与类风湿关节炎严重程度相关。此外，类风湿关节炎组和对照组之间铜和锌水平未发现显著差异。脂联素、维生素D、铜和锌在类风湿关节炎发展中的明确作用尚未完全确定。基于疾病活动度，这些参数可以调节炎症状态，因此它们有可能作为有前景的治疗生物标志物，用于跟踪疾病严重程度以及类风湿关节炎患者的病情进展和治疗效果。
