Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico.
Departamento de Tecnologías Avanzadas, Unidad Profesional Interdisciplinaria en Ingeniería y Tecnologías Avanzadas del Instituto Politécnico Nacional, Mexico City, Mexico.
Virus Res. 2019 Jun;266:1-14. doi: 10.1016/j.virusres.2019.03.015. Epub 2019 Mar 28.
Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naïve C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.
外泌体是大多数细胞类型分泌到细胞外空间的内体起源的小膜囊泡。病毒感染细胞释放的外泌体可以介导细胞间通讯，促进或调节病毒传播。登革热病毒（DENV）是一种由伊蚊叮咬传播给人类的虫媒病毒。有趣的是，外泌体在哺乳动物细胞中感染 DENV 中的作用已经被描述。然而，关于源自感染蚊子细胞的外泌体知之甚少。因此，从 DENV 感染的 C6/36 细胞中分离、纯化并分析了用针对人四跨膜蛋白 CD9 的抗体标记的外泌体，该抗体显示与人 CD9 的同源物有交叉反应，而人 CD9 的同源物存在于白纹伊蚊（AalCD9）中。来自 DENV 感染细胞的外泌体比来自未感染细胞的外泌体大，含有类病毒颗粒，并且能够感染未感染的 C6/36 细胞，这表明外泌体在病毒传播中发挥作用。
