University of Agricultural Sciences, Bangalore, 560065, India.
ICAR-AICRP on Post Harvest Technology, UAS, GKVK, Bengaluru, 560065, India.
BMC Microbiol. 2023 Jul 7;23(1):179. doi: 10.1186/s12866-023-02922-w.
Over 380 host plant species have been known to develop leaf spots as a result of the fungus Alternaria alternata. It is an aspiring pathogen that affects a variety of hosts and causes rots, blights, and leaf spots on different plant sections. In this investigation, the lipopeptides from the B. subtilis strains T3, T4, T5, and T6 were evaluated for their antifungal activities. In the genomic DNA, iturin, surfactin, and fengycin genes were found recovered from B. subtilis bacterium by PCR amplification. From different B. subtilis strains, antifungal Lipopeptides were extracted, identified by HPLC, and quantified with values for T3 (24 g/ml), T4 (32 g/ml), T5 (28 g/ml), and T6 (18 g/ml). To test the antifungal activity, the isolated lipopeptides from the B. subtilis T3, T4, T5, and T6 strains were applied to Alternaria alternata at a concentration of 10 g/ml. Lipopeptides were found to suppress Alternaria alternata at rates of T3 (75.14%), T4 (75.93%), T5 (80.40%), and T6 (85.88%). The T6 strain outperformed the other three by having the highest antifungal activity against Alternaria alternata (85.88%).
已有超过 380 种宿主植物因链格孢菌（Alternaria alternata）而产生叶斑病。它是一种有抱负的病原体，会影响多种宿主，并导致不同植物部位的腐烂、枯萎和叶斑病。在这项研究中，评估了枯草芽孢杆菌菌株 T3、T4、T5 和 T6 的脂肽的抗真菌活性。通过 PCR 扩增，从枯草芽孢杆菌的基因组 DNA 中发现回收了iturin、表面活性剂和fengycin 基因。从不同的枯草芽孢杆菌菌株中提取了抗真菌脂肽，通过 HPLC 进行了鉴定，并通过 T3（24 g/ml）、T4（32 g/ml）、T5（28 g/ml）和 T6（18 g/ml）的值进行了定量。为了测试抗真菌活性，将枯草芽孢杆菌 T3、T4、T5 和 T6 菌株分离的脂肽以 10 g/ml 的浓度应用于链格孢菌。发现脂肽以 T3（75.14%）、T4（75.93%）、T5（80.40%）和 T6（85.88%）的抑制率抑制链格孢菌。T6 菌株对链格孢菌（85.88%）的抗真菌活性最高，优于其他三种菌株。
