从伊拉克巴格达市捕获的大鼠（）内脏中分离细菌。
Bacterial isolation from internal organs of rats () captured in Baghdad city of Iraq.
作者信息
Ayyal Nagham Mohammed, Abbas Zainab Abdulzahra, Karim Abdulkarim Jafar, Abbas Zainab Majid, Al-Salihi Karima Akool, Khalaf Jenan Mahmood, Mahmood Dunya Dhafir, Mohammed Eman Abdullah, Jumaa Rawaa Saladdin, Abdul-Majeed Dhuha Ismaeel
机构信息
Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Department of Pathological Analysis, Babylon Technical Institute, Al-Furat Al-Awsat Technical University, Babylon, Iraq.
出版信息
Vet World. 2019 Jan;12(1):119-125. doi: 10.14202/vetworld.2019.119-125. Epub 2019 Jan 22.
AIM
Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq.
MATERIALS AND METHODS
A total of 120 black rats () were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were used to detect 157:7 and spp., respectively. Biochemical tests on analytical profile index, microscopic examination, and commercial kit for latex agglutination test for serotyping O157:H7 were used.
RESULTS
Mixed bacterial isolates were recorded as 116, 52, 36, 28, 18, 6, and 4 from intestinal contents, deep pharyngeal, liver and spleen, urine, lung, brain, and kidney, respectively. Microorganisms included , , spp., spp., , , , , , spp., spp., , spp., spp., , , spp., spp., , , and spp. The highest bacterial prevalence (88; 73.33%) was recorded for , where 68 isolates were identified from the intestinal contents. Of these, four isolates were O157:H7.
CONCLUSION
Rats are important carriers and transmitters of a number of pathogens and can disseminate these microorganisms to humans and animals.
目的
老鼠被指控传播多种人畜共患疾病。本研究旨在从伊拉克巴格达市捕获的老鼠内脏中分离和鉴定细菌。
材料与方法
从巴格达市不同区域共捕获120只黑鼠（ ）。在安乐死之前，将老鼠单独饲养在塑料笼中3小时。无菌采集深部咽拭子、肠内容物、尿液以及肝脏、脾脏、肺、肾脏和脑的组织块。将标本接种到蛋白胨水中，于37°C培养24小时进行增菌。然后将每个标本的一环接种到麦康凯琼脂、血琼脂和甘露醇盐琼脂上。分别使用CHROMagar O157 H7和CHROMagar Listeria检测 157:7和 属细菌。采用分析谱指数生化试验、显微镜检查以及用于O157:H7血清分型的乳胶凝集试验商业试剂盒。
结果
从肠内容物、深部咽、肝脏和脾脏、尿液、肺、脑和肾脏中分别记录到混合细菌分离株116株、52株、36株、28株、18株、6株和4株。微生物包括 、 、 属、 属、 、 、 、 、 、 属、 属、 、 属、 属、 、 、 属、 属、 、 属。 的细菌感染率最高（88株；73.33%），其中68株分离自肠内容物。其中，4株分离株为O157:H7。
结论
老鼠是多种病原体的重要携带者和传播者，可将这些微生物传播给人类和动物。
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