Priest N D, Jackson S
Int J Radiat Biol Relat Stud Phys Chem Med. 1978 Jul;34(1):49-65. doi: 10.1080/09553007814550621.
Male and female hamsters and a female rabbit were injected with 241Pu citrate. The hamsters were killed serially at 15 min, 2 hours, 1 day and 10 days after injection, and the rabbit 1 week after injection. The gonads were examined for 241Pu by tissue-section autoradiography. Soon after injection the plutonium was concentrated by the contents of atretic Graafian follicles and by thecal rings in the ovary, but was found to be dispersed throughout the testes. It is suggested that the disperse distribution in the testes which is only seen soon after injection may be an artefact of tissue processing. One day after injection, plutonium was accumulated by macrophages in both the follicles of the ovary and in the interstitial tissue of the testes. Macrophages containing plutonium later migrated away from the aretic ovarian follicles towards the ovarian medulla. This pattern of distribution and redistribution in the ovary is regarded as likely to lower the effective dose from a-emitting plutonium isotopes to the viable oocytes. No migration of macrophages was seen in the testes. Histochemical staining methods revealed the presence of acid protoglycans, including chondroitin sulphate, and glycoproteins at the sites of plutonium concentration in the ovary. These molecules are regarded as likely receptor sites for plutonium. In the testes no acidic carbohydrates were found, and it is suggested that the initial binding site for plutonium may be a compound lipid. This was deduced from the apparent inability of the interstitial tissue of the testes to bind plutonium in situ.
给雄性和雌性仓鼠以及一只雌性兔子注射柠檬酸钚-241。仓鼠在注射后15分钟、2小时、1天和10天依次处死，兔子在注射后1周处死。通过组织切片放射自显影检查性腺中的钚-241。注射后不久，钚集中在卵巢中闭锁卵泡的内容物和卵泡膜环中，但在睾丸中发现其分布分散。有人认为，仅在注射后不久在睾丸中看到的分散分布可能是组织处理的假象。注射后一天，钚在卵巢卵泡和睾丸间质组织中的巨噬细胞中积累。含钚的巨噬细胞后来从闭锁的卵巢卵泡向卵巢髓质迁移。卵巢中的这种分布和再分布模式被认为可能会降低发射α粒子的钚同位素对存活卵母细胞的有效剂量。在睾丸中未观察到巨噬细胞的迁移。组织化学染色方法显示，在卵巢中钚浓度较高的部位存在酸性蛋白聚糖，包括硫酸软骨素和糖蛋白。这些分子被认为可能是钚的受体位点。在睾丸中未发现酸性碳水化合物，有人认为钚的初始结合位点可能是一种复合脂质。这是从睾丸间质组织在原位结合钚的明显无能推断出来的。
