Lin Guo-Hua, Lin Li-Zhu, Zhang Ying, Pei Wen-Ya, Zeng Jing-Chun, Zhao Wei-Xuan
First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
People's Hospital of Yuxi City, Yuxi 653000, Yunnan Province.
Zhen Ci Yan Jiu. 2019 Feb 25;44(2):136-9. doi: 10.13702/j.1000-0607.170933.
To observe the influence of fire needle stimulation at Sihua acupoints combined with chemotherapy on Th1/Th2 imbalance and performance status in the patients with advanced non-small cell lung cancer (NSCLC) so as to investigate its immunological mechanism underlying the improvement of lung cancer.
Sixty eligible NSCLC inpatients were randomly assigned to a control group and a treatment group (＝30 cases in each one). The control group was treated with paclitaxel combined with cisplatin (TP) or gemcitabine plus cisplatin (GP) of chemotherapy, and the treatment group was treated with fire needle stimulation at Sihua acupoints (i.e. bilateral Geshu [BL17] and Danshu [BL19]) and with chemotherapy simultaneously. The fire needle stimulation was given once daily for successive 7 days. One day before and 14 days after chemotherapy, the patients' blood samples were collected for assaying the concentrations of Th1 related cytokines (IL-2, IFN-γ) and Th2 related cytokines (IL-4, IL-10) with ELISA. The Karnofsky performance status (KPS) score was used to assess the patient's physical conditions before and 14 days after chemotherapy.
Compared with the results before treatment, the levels of IL-2 and IFN-γ concentration increased obviously and the levels of IL-4 and IL-10 concentration as well as KPS score decreased significantly in the treatment group and the control group after treatment (all <0.01). After treatment, compared with the control group, the levels of IL-2 and IFN-γ concentration in the treatment group increased and the levels of IL-4 and IL-10 concentration decreased (<0.01); and the decrease amplitude of KPS score in the treatment group was less than that in the control group (<0.01).
Fire needle stimulation at Sihua acupoints improves the function of Th1 cells, and reduces the function of Th2 cells. As a result, the balance between Th1 and Th2 has been modified and the deterioration degree of the performance status been alleviated in the patients after chemotherapy.
观察四花穴火针刺激联合化疗对晚期非小细胞肺癌（NSCLC）患者Th1/Th2失衡及生活质量的影响，以探讨其改善肺癌的免疫机制。
将60例符合条件的NSCLC住院患者随机分为对照组和治疗组（每组30例）。对照组采用紫杉醇联合顺铂（TP）或吉西他滨联合顺铂（GP）化疗，治疗组采用四花穴（即双侧膈俞[BL17]和胆俞[BL19]）火针刺激并同时化疗。火针刺激每天1次，连续7天。化疗前1天和化疗后14天采集患者血样，采用酶联免疫吸附测定法（ELISA）检测Th1相关细胞因子（IL-2、IFN-γ）和Th2相关细胞因子（IL-4、IL-10）的浓度。采用卡氏功能状态（KPS）评分评估化疗前及化疗后14天患者的身体状况。
治疗后，治疗组和对照组IL-2和IFN-γ浓度水平明显升高，IL-4和IL-10浓度水平及KPS评分均显著降低（均P<0.01）。治疗后，与对照组比较，治疗组IL-2和IFN-γ浓度水平升高，IL-4和IL-10浓度水平降低（P<0.01）；治疗组KPS评分下降幅度小于对照组（P<0.01）。
四花穴火针刺激可改善Th1细胞功能，降低Th2细胞功能，从而调节Th1与Th2的平衡，减轻化疗后患者生活质量的恶化程度。
