Research Centre for Smart Wearable Technology, Institute of Textiles and Clothing, Hong Kong Polytechnic University, Hong Kong, China.
Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
Macromol Biosci. 2019 May;19(5):e1800432. doi: 10.1002/mabi.201800432. Epub 2019 Apr 5.
In this work, it is first reported that the poly (3-hydroxybutyric acid) (PHB) oligomer with a few degrees of polymerization possesses effective antibacterial and antifungal properties. Two preparation methods for the PHB oligomer are described, namely, one-step ring-opening polymerization of β-butyrolactone and extraction from the fermented PHB polymer. An appropriate amount of the synthesized PHB oligomer shows no physiological toxicity to the skin and major organs of mice. Topological application of the synthesized PHB oligomer imparts antimicrobial ability to non-antibacterial fabrics with washing resistance. The synthesized PHB oligomer offers effective sterilization and promotes wound healing in infected nude mice. Most importantly, the PHB oligomer is also reactive to drug-resistant bacteria. These results suggest that the PHB oligomer is not only a great candidate for antimicrobial modification but also a promising one for biomedical applications. Finally, the antimicrobial mechanisms of the PHB oligomer are revealed, and these include disruption of biofilm and the bacterial wall/membrane, leakage of the intracellular content, inhibition of protein activity, and change in the transmembrane potential.
在这项工作中，首次报道了具有少数聚合度的聚（3-羟基丁酸）（PHB）低聚物具有有效的抗菌和抗真菌性能。描述了两种 PHB 低聚物的制备方法，即β-丁内酯的一步开环聚合和从发酵的 PHB 聚合物中提取。适量合成的 PHB 低聚物对小鼠的皮肤和主要器官没有生理毒性。合成的 PHB 低聚物的拓扑应用赋予耐洗性的非抗菌织物抗菌能力。合成的 PHB 低聚物可有效杀菌并促进感染裸鼠的伤口愈合。最重要的是，PHB 低聚物对耐药菌也有反应。这些结果表明，PHB 低聚物不仅是抗菌修饰的理想候选物，而且也是生物医学应用的有前途的候选物。最后，揭示了 PHB 低聚物的抗菌机制，包括破坏生物膜和细菌细胞壁/膜、细胞内内容物泄漏、抑制蛋白质活性以及改变跨膜电位。
