酶联免疫吸附测定法与放射免疫沉淀试验在检测鼻分泌物中肺炎支原体免疫球蛋白A抗体方面的比较
Comparison of enzyme-linked immunosorbent assay and radioimmunoprecipitation test for detection of immunoglobulin A antibodies to Mycoplasma pneumoniae in nasal secretions.
作者信息
Naot Y, Lis E, Siman-Tov R, Brunner H
出版信息
J Clin Microbiol. 1986 Nov;24(5):892-3. doi: 10.1128/jcm.24.5.892-893.1986.
Abstract
An immunoglobulin A (IgA) enzyme-linked immunosorbent assay was developed and compared with the radioimmunoprecipitation test in determinations of IgA antibodies to Mycoplasma pneumoniae. Of 45 nasal secretions obtained from infected volunteers, 42 (93.4%) were positive and 2 (4.4%) were negative in both tests. The IgA enzyme-linked immunosorbent assay is at least as sensitive as the IgA radioimmunoprecipitation test but is simpler and safer and should therefore be preferred.
摘要
开发了一种免疫球蛋白A（IgA）酶联免疫吸附测定法，并将其与放射免疫沉淀试验在肺炎支原体IgA抗体测定中进行比较。在从感染志愿者获得的45份鼻分泌物中，两种检测方法均呈阳性的有42份（93.4%），呈阴性的有2份（4.4%）。IgA酶联免疫吸附测定法至少与IgA放射免疫沉淀试验一样灵敏，但更简便、安全，因此应优先选用。
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