Salesa Beatriz, Martí Miguel, Frígols Belén, Serrano-Aroca Ángel
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, c/Guillem de Castro 94, 46001 Valencia, Spain.
Polymers (Basel). 2019 Mar 10;11(3):453. doi: 10.3390/polym11030453.
Due to the current global health problem of antibiotic resistant recently announced by the World Health Organization, there is an urgent necessity of looking for new alternative antibacterial materials able to treat and impede multidrug-resistant infections which are cost-effective and non-toxic for human beings. In this regard, carbon nanofibers (CNFs) possess currently much lower cost than other carbon nanomaterials, such as graphene oxide, and exhibit excellent chemical, mechanical and electric properties. Furthermore, here, the first report on the antibacterial activity of CNFs was demonstrated. Thus, these nanomaterials, in pure form or incorporated in a minuscule amount into calcium alginate composite films to reduce production costs as much as possible, showed to be new weapons against a globally spreading multidrug-resistant pathogen, the methicillin-resistant (MRSE). This Gram-positive bacterium is becoming one of the most dangerous pathogens, due to its abundance on skin. In this study, these hollow filamentous materials, in direct contact with cells and loaded in the low-cost calcium alginate composite films, showed no cytotoxicity for human keratinocyte HaCaT cells, which render them very promising for biomedical applications. The CNFs used in this work were characterized by Raman spectroscopy and observed by high-resolution transmission electron with energy-disperse X-ray spectroscopy.
由于世界卫生组织最近宣布的当前全球抗生素耐药性健康问题，迫切需要寻找能够治疗和阻止耐多药感染的新型替代抗菌材料，这些材料对人类具有成本效益且无毒。在这方面，碳纳米纤维（CNF）目前的成本比其他碳纳米材料（如氧化石墨烯）低得多，并具有优异的化学、机械和电学性能。此外，本文首次报道了CNF的抗菌活性。因此，这些纳米材料，无论是纯形式还是以极少量掺入海藻酸钙复合膜中以尽可能降低生产成本，都显示出是对抗全球传播的耐多药病原体——耐甲氧西林金黄色葡萄球菌（MRSE）的新武器。这种革兰氏阳性细菌因其在皮肤上的大量存在而正成为最危险的病原体之一。在本研究中，这些中空丝状材料与细胞直接接触并负载于低成本的海藻酸钙复合膜中，对人角质形成细胞HaCaT细胞无细胞毒性，这使其在生物医学应用中极具前景。本研究中使用的CNF通过拉曼光谱进行表征，并通过高分辨率透射电子显微镜和能量色散X射线光谱进行观察。
