Department of Anatomy, Laboratory of Molecular Reproduction & Genetics, All India Institute of Medical Sciences, New Delhi, India.
Department of Obstetrics & Gynaecology, All India Institute of Medical Sciences, New Delhi, India.
Indian J Med Res. 2018 Dec;148(Suppl):S134-S139. doi: 10.4103/ijmr.IJMR_1988_17.
BACKGROUND & OBJECTIVES: Recurrent pregnancy loss (RPL) is one of the devastating complications of pregnancy and current focus lies in addressing the management of paternal factors. Dysregulation in selective transcripts delivered to oocyte at fertilization can result in pregnancy losses and adversely affect embryogenesis. The objective of this study was to assess the effect of yoga-based lifestyle intervention (YBLI) on seminal oxidative stress (OS), DNA damage and spermatozoal transcript levels.
The present study was a part of a prospective ongoing exploratory study and 30 male partners of couples with RPL were included from August 2016 to June 2017. Semen samples were obtained at baseline and at the end of YBLI (21 days). Gene expression analysis was performed by quantitative polymerase chain reaction on spermatozoal FOXG1, SOX3, OGG1, PARP1, RPS6, RBM9, RPS17 and RPL29. The levels of seminal OS and sperm DNA damage was assessed by measuring levels of reactive oxygen species (ROS) by chemiluminescence and DNA fragmentation index (DFI) by sperm chromatin structure assay.
SOX3, OGG1 and PARP1 were observed to be upregulated, while FOXG1, RPS6, RBM9, RPS17 and RPL29 showed downregulation. A significant reduction in ROS levels, an increase in sperm motility, sperm count (done twice) and a decrease in DFI was seen after YBLI.
INTERPRETATION & CONCLUSIONS: Adopting YBLI may help in a significant decline in oxidative DNA damage and normalization of sperm transcript levels. This may not only improve pregnancy outcomes but also improve the health trajectory of the offspring.
复发性流产（RPL）是妊娠的一种严重并发症，目前的重点在于解决父系因素的处理。受精时向卵母细胞传递的选择性转录本的失调可导致妊娠丢失，并对胚胎发生产生不利影响。本研究的目的是评估瑜伽为基础的生活方式干预（YBLI）对精液氧化应激（OS）、DNA 损伤和精子转录本水平的影响。
本研究是一项前瞻性探索性研究的一部分，纳入了 2016 年 8 月至 2017 年 6 月期间 30 对 RPL 夫妇的男性伴侣。在基线和 YBLI 结束时（21 天）获取精液样本。通过定量聚合酶链反应（qPCR）在精子 FOXG1、SOX3、OGG1、PARP1、RPS6、RBM9、RPS17 和 RPL29 上进行基因表达分析。通过化学发光法测量活性氧（ROS）水平和精子染色质结构试验（SCSA）评估精子 DNA 损伤的程度，来评估精液 OS 和精子 DNA 损伤的水平。
SOX3、OGG1 和 PARP1 被观察到上调，而 FOXG1、RPS6、RBM9、RPS17 和 RPL29 则下调。YBLI 后，ROS 水平显著降低，精子活力、精子计数（两次）增加，DFI 降低。
采用 YBLI 可能有助于显著降低氧化 DNA 损伤并使精子转录本水平正常化。这不仅可以改善妊娠结局，还可以改善后代的健康轨迹。
