Food Control Laboratory, Agronomic Research Institute, BP1163, Lomé, Togo.
Department of Animal Physiology, Lomé University, BP 1515, Lomé, Togo.
Mycotoxin Res. 2019 Nov;35(4):321-327. doi: 10.1007/s12550-019-00351-1. Epub 2019 Apr 9.
Mycotoxins are fungal secondary metabolites frequently affecting agronomical crops and consequently imposing a major challenge for food safety and public health. In this study, a total of 67 raw cereals (55 maize and 12 sorghum) were collected from the market of Togo. The samples were investigated on the occurrence of 21 mycotoxins using state-of-the-art high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The most frequent occurring mycotoxins were fumonisins (88 and 67% for maize and sorghum respectively) with concentrations ranging from 101 to 1838 μg/kg for maize and 81.5 to 361 μg/kg for sorghum, respectively. Aflatoxin B1 was detected in 38% of the maize samples with maximum contamination levels of 256 μg/kg, and 25% of the sorghum samples (range 6-16 μg/kg). The concentrations of aflatoxins were high in maize, with some cases exceeding the maximum legislative limits (EU) for unprocessed maize placed on the market. In addition to these high contamination levels, the co-occurrence of three classes of mycotoxins (i.e., aflatoxins, fumonisins, and trichothecenes) was observed in this study. For the first time, the multi-mycotoxins occurrence in agronomical crops in Togo was reported.
真菌毒素是真菌的次生代谢产物，常影响农作物，对食品安全和公共健康构成重大挑战。本研究共采集来自多哥市场的 67 份未经加工的原始谷物（55 份玉米和 12 份高粱）样本，采用最先进的高效液相色谱-串联质谱法（HPLC-MS/MS）检测 21 种真菌毒素的存在情况。结果显示，玉米和高粱中最常出现的真菌毒素分别为伏马菌素（分别为 88%和 67%），浓度范围分别为 101-1838μg/kg 和 81.5-361μg/kg。本研究中，38%的玉米样本中检测到黄曲霉毒素 B1，最高污染水平为 256μg/kg，25%的高粱样本中（范围为 6-16μg/kg）检测到黄曲霉毒素 B1。玉米中黄曲霉毒素的浓度较高，有些情况超过了（欧盟）未加工玉米投放市场的最大限量规定。除了这些高污染水平外，本研究还观察到三类真菌毒素（即黄曲霉毒素、伏马菌素和单端孢霉烯族毒素）同时存在。本研究首次报道了多哥农业作物中多类真菌毒素的存在情况。
