Hunter S W, McNeil M R, Brennan P J
J Bacteriol. 1986 Nov;168(2):917-22. doi: 10.1128/jb.168.2.917-922.1986.
A diglycosyl diacylglycerol was isolated from Mycobacterium tuberculosis, and its structure was established by a combination of methylation analysis, 1H nuclear magnetic resonance, and fast atom bombardment-mass spectrometry. It is a 1,2-diacyl-[beta-D-glucopyranosyl(1"----6')-beta-D-glucopyranosyl(1'---- 3)]- sn-glycerol and exists in at least five molecular species differing in fatty acyl substituents. The major constituent fatty acids were identified as iso- and anteisopentadecanoate, iso- and n-hexadecanoate, and iso- and anteisoheptadecanoate. Although glycosyl diacylglycerols are common membrane components of gram-positive bacteria, this report represents the first substantial evidence for the presence of a glycosyl diacylglycerol within a member of the Mycobacterium genus. Although the glycolipid is not a major component of M. tuberculosis, it reacts readily in enzyme-linked immunosorbent assay against rabbit antibodies raised against whole bacteria and thus may be useful for the serodiagnosis of tuberculosis.
从结核分枝杆菌中分离出一种二糖基二酰基甘油，并通过甲基化分析、氢核磁共振和快原子轰击质谱相结合的方法确定了其结构。它是一种1,2-二酰基-[β-D-吡喃葡萄糖基(1″→6′)-β-D-吡喃葡萄糖基(1′→3)]-sn-甘油，并且至少以五种脂肪酸酰基取代基不同的分子形式存在。主要的组成脂肪酸被鉴定为异十五烷酸、反异十五烷酸、异十六烷酸、正十六烷酸、异十七烷酸和反异十七烷酸。虽然糖基二酰基甘油是革兰氏阳性菌常见的膜成分，但本报告首次提供了实质性证据，证明在分枝杆菌属的一个成员中存在糖基二酰基甘油。虽然这种糖脂不是结核分枝杆菌的主要成分，但它在酶联免疫吸附试验中很容易与针对全菌产生的兔抗体发生反应，因此可能对结核病的血清诊断有用。
