Yang Zerui, Huang Yuying, An Wenli, Zheng Xiasheng, Huang Song, Liang Lingling
DNA Barcoding Laboratory for TCM Authentication, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Pharmaceutical School, YouJiang Medical University for Nationalities, Baise 533000, China.
Plants (Basel). 2019 Apr 3;8(4):87. doi: 10.3390/plants8040087.
Mill, an important Chinese herbal medicine, is widely used as a dietary supplement and food. Here the chloroplast (CP) genome of was sequenced and analyzed, revealing a size of 155,756 bp and with a 37.8% GC content. The CP genome comprises a large single copy region (LSC) of 86,595 bp and a small single copy region (SSC) of 18,209 bp, and two inverted repeat regions (IRa and IRb) of 25,476 bp separated by the single copy regions. The genome encodes 114 genes, 16 of which are duplicated. Most of the 85 protein-coding genes (CDS) had standard ATG start codons, while 3 genes including , and had abnormal start codons (ACT and ACG). In addition, a strong A/T bias was found in the majority of simple sequence repeats (SSRs) detected in the CP genome. Analysis of the phylogenetic relationships among 16 species revealed that is a sister taxon to . Overall, the complete sequence and annotation of the CP genome provides valuable genetic information to facilitate precise understanding of the taxonomy, species and phylogenetic evolution of the Solanaceae family.
枸杞是一种重要的中药材，被广泛用作膳食补充剂和食品。本文对枸杞叶绿体（CP）基因组进行了测序和分析，结果显示其大小为155,756 bp，GC含量为37.8%。枸杞CP基因组包括一个86,595 bp的大单拷贝区域（LSC）、一个18,209 bp的小单拷贝区域（SSC）以及两个由单拷贝区域隔开的25,476 bp的反向重复区域（IRa和IRb）。该基因组编码114个基因，其中16个是重复的。85个蛋白质编码基因（CDS）中的大多数具有标准的ATG起始密码子，而包括、和在内的3个基因具有异常起始密码子（ACT和ACG）。此外，在CP基因组中检测到的大多数简单序列重复（SSR）中发现了强烈的A/T偏向。对16个物种的系统发育关系分析表明，枸杞是枸杞的姐妹分类群。总体而言，枸杞CP基因组的完整序列和注释提供了有价值的遗传信息，有助于准确了解茄科的分类学、物种和系统发育进化。
