Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
Biomed Res Int. 2021 Jan 28;2021:8826586. doi: 10.1155/2021/8826586. eCollection 2021.
Sudachitin, which is a polymethoxylated flavonoid found in the peel of Citrus sudachi, has some biological activities. However, the effect of sudachitin on periodontal resident cells is still uncertain. The aim of this study was to examine if sudachitin could decrease the expression of inflammatory mediators such as cytokines, chemokines, or matrix metalloproteinase (MMP) in interleukin- (IL-) 1-stimulated human periodontal ligament cells (HPDLC). Sudachitin inhibited IL-1-induced IL-6, IL-8, CXC chemokine ligand (CXCL)10, CC chemokine ligand (CCL)2, MMP-1, and MMP-3 production in HPDLC. On the other hand, tissue inhibitor of metalloproteinase- (TIMP-) 1 expression was increased by sudachitin treatment. Moreover, we found that the nuclear factor- (NF-) B and protein kinase B (Akt) pathways in the IL-1-stimulated HPDLC were inhibited by sudachitin treatment. These findings indicate that sudachitin is able to reduce inflammatory mediator production in IL-1-stimulated HPDLC by inhibiting NF-B and Akt pathways.
川陈皮素是一种在柑橘属柚子皮中发现的多甲氧基类黄酮，具有一些生物活性。然而，川陈皮素对牙周常驻细胞的影响尚不清楚。本研究旨在探讨川陈皮素是否能降低白细胞介素 (IL-) 1 刺激的人牙周膜细胞 (HPDLC) 中炎症介质（如细胞因子、趋化因子或基质金属蛋白酶 (MMP)）的表达。川陈皮素抑制了 IL-1 诱导的 HPDLC 中 IL-6、IL-8、CXC 趋化因子配体 (CXCL)10、CC 趋化因子配体 (CCL)2、MMP-1 和 MMP-3 的产生。另一方面，川陈皮素处理增加了基质金属蛋白酶抑制剂 (TIMP-) 1 的表达。此外，我们发现川陈皮素处理抑制了 IL-1 刺激的 HPDLC 中的核因子 (NF-) B 和蛋白激酶 B (Akt) 通路。这些发现表明，川陈皮素通过抑制 NF-B 和 Akt 通路，能够减少 IL-1 刺激的 HPDLC 中炎症介质的产生。
