碱基编辑器的开发与应用。

Development and Application of Base Editors.

机构信息

1 Shanghai Institute for Advanced Immunochemical Studies and Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

2 Chinese Academy of Sciences (CAS) Key Laboratory of Computational Biology, CAS-Max Planck Gesellschaft Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

出版信息

CRISPR J. 2019 Apr;2(2):91-104. doi: 10.1089/crispr.2019.0001.

DOI:10.1089/crispr.2019.0001

PMID:30998092

Abstract

Base editing is emerging as a potent new strategy to achieve precise gene editing. By combining different nucleobase deaminases with Cas9 or Cpf1 proteins, several base editors have recently been developed to achieve targeted base conversions in different genomic contexts. Importantly, base editors have been successfully applied in animals, plants, and bacteria to induce precise substitutions at the single-base level with high efficiency. In this review, we summarize recent progress in the development and application of base editors and discuss some of the future directions of the technology.

摘要

碱基编辑技术作为一种新兴的精确基因编辑策略正在不断发展。通过将不同的碱基脱氨酶与 Cas9 或 Cpf1 蛋白相结合，最近已经开发出了几种碱基编辑器，可在不同的基因组环境中实现靶向碱基转换。重要的是，碱基编辑器已成功应用于动物、植物和细菌中，可高效地在单碱基水平上诱导精确的替换。在本文中，我们总结了碱基编辑器的开发和应用的最新进展，并讨论了该技术的一些未来发展方向。