通过腺嘌呤和胞嘧啶碱基编辑实现精确基因组工程。
Precision genome engineering through adenine and cytosine base editing.
机构信息
Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea.
Department of Chemistry, Seoul National University, Seoul, Republic of Korea.
出版信息
Nat Plants. 2018 Mar;4(3):148-151. doi: 10.1038/s41477-018-0115-z. Epub 2018 Feb 26.
Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically impaired CRISPR-Cas9 variant, are powerful new tools for targeted base editing in cells and organisms. Together with cytosine base editors (CBEs), ABEs enable single-nucleotide conversions cleanly, efficiently and reversibly without double-stranded DNA cleavage, advancing genome editing in a new dimension.
腺嘌呤碱基编辑器（ABEs）由一种工程化的脱氨酶和一种催化失活的 CRISPR-Cas9 变体组成，是在细胞和生物体内进行靶向碱基编辑的强大新工具。ABEs 与胞嘧啶碱基编辑器（CBEs）一起，可以在不进行双链 DNA 切割的情况下，高效、可逆地实现单核苷酸转换，将基因组编辑推进到一个新的维度。
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