Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
Clinic of Gynecology and Urogynecology, Pomeranian Medical University, 70-204 Szczecin, Poland.
Molecules. 2019 Apr 17;24(8):1508. doi: 10.3390/molecules24081508.
According to a review of the literature, there is a lack of data on the mechanisms that participate in the suppression of inflammation that accompanies polycystic ovary syndrome (PCOS). Additionally, the changes in oxidative status resulting from a low-calorie diet have not been studied in a group of women with PCOS, and the oxidation and reduction processes associated with PCOS have not been explained.
The study involved 49 women who were diagnosed with PCOS according to Rotterdam's criteria, and 24 women voluntarily agreed to a three-month dietary intervention. The dietary intervention was carried out for 3 months. Glutathione peroxidase (GPx3) activity, the Ferric reducing ability of plasma, and uric acid concentration were measured spectrophotometrically both before and after the intervention. Statistical analysis was performed with the Statistica 10.0 software package, and a Pearson's correlation matrix was generated.
A lower concentration of GPx3 was observed in women with PCOS (before the dietetic intervention began) compared with the GPx3 levels in healthy women. A relationship was shown between GPx3 levels and the concentration of prolactin, insulin on fasting, and triglycerides. After the dietary intervention, increases in uric acid and GPx3 activity were noted, as well as numerous relationships between anthropometric and biochemical parameters. The ferric reducing/antioxidant power did not change significantly.
Inhibiting the effect of prolactin (by the level of reactive oxygen species) on the activity of GPx3 could be a starting point for the increase in antioxidative stress and the development of the inflammatory state associated with PCOS pathophysiology. Following a low-calorie diet with a lower glycemic index is proposed to silence inflammation by increasing the concentration of uric acid. During GPx3 mobilization, women with PCOS have a higher demand for selenium, and its deficiencies may contribute to disordered thyroid hormone synthesis. The three-month dietary intervention did not silence redox processes in the examined group of women.
根据文献综述，多囊卵巢综合征（PCOS）伴随的炎症抑制机制的数据尚缺乏。此外，低热量饮食引起的氧化状态变化在 PCOS 患者群体中尚未得到研究，与 PCOS 相关的氧化和还原过程也未得到解释。
该研究纳入了 49 名根据 Rotterdam 标准诊断为 PCOS 的女性和 24 名自愿接受为期三个月饮食干预的女性。进行了为期 3 个月的饮食干预。干预前后，使用分光光度法测量谷胱甘肽过氧化物酶（GPx3）活性、血浆铁还原能力和尿酸浓度。使用 Statistica 10.0 软件包进行统计分析，并生成 Pearson 相关矩阵。
与健康女性相比，PCOS 女性（在进行饮食干预之前）的 GPx3 浓度较低。GPx3 水平与催乳素、空腹胰岛素和甘油三酯浓度之间存在相关性。饮食干预后，尿酸和 GPx3 活性增加，并且许多人体测量学和生化参数之间存在关系。铁还原/抗氧化能力没有显著变化。
抑制催乳素（通过活性氧水平）对 GPx3 活性的影响可能是增加抗氧化应激和发展与 PCOS 病理生理学相关的炎症状态的起点。建议采用低热量、低血糖指数的饮食来沉默炎症，增加尿酸浓度。在 GPx3 动员期间，PCOS 女性对硒的需求更高，其缺乏可能导致甲状腺激素合成紊乱。在研究的女性群体中，为期三个月的饮食干预并没有沉默氧化还原过程。
