Zhou Qianmei, Zhang Weihong, Li Tian, Tang Runwei, Li Chaoran, Yuan Shuai, Fan Desheng
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Breast Surgery Department, Baoshan Branch, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201900, China.
Evid Based Complement Alternat Med. 2019 Mar 17;2019:9610629. doi: 10.1155/2019/9610629. eCollection 2019.
Formononetin, an active ingredient isolated from the traditional Chinese medicinal herb , has anticancer and chemoresistance-reducing biological activities. We evaluated the efficacy of formononetin in improving the tumoricidal effect of everolimus by suppressing the mTOR pathway in breast cancer cells.
Cell survival was assessed using an MTT assay. Apoptosis was detected using flow cytometry. Proteins related to the mTOR pathway were detected and assessed using real-time PCR and Western blot analysis The results showed that formononetin enhances the efficacy of everolimus in suppressing breast cancer cell growth both in vitro and in vivo. The combination of formononetin and everolimus resulted in a 2-fold decrease in tumor volume and a 21.6% decrease in cell survival. The apoptosis ratio in cells treated with formononetin and everolimus increased by 27.9%. Formononetin and everolimus also inhibited the expression of p-mTOR and p-P70S6K and increased the expression of PTEN and p-4EBP-1. Notably, formononetin alone inhibited p-Akt expression but not everolimus.
Formononetin enhances the tumoricidal effect of everolimus by inhibiting the activity of Akt.
芒柄花黄素是从传统中草药中分离出的一种活性成分，具有抗癌和降低化疗耐药性的生物活性。我们评估了芒柄花黄素通过抑制乳腺癌细胞中的mTOR通路来提高依维莫司杀瘤效果的功效。
使用MTT法评估细胞存活率。采用流式细胞术检测细胞凋亡。使用实时PCR和蛋白质免疫印迹分析检测并评估与mTOR通路相关的蛋白质。结果表明，芒柄花黄素在体外和体内均可提高依维莫司抑制乳腺癌细胞生长的功效。芒柄花黄素与依维莫司联合使用可使肿瘤体积减少两倍，细胞存活率降低21.6%。用芒柄花黄素和依维莫司处理的细胞凋亡率增加了27.9%。芒柄花黄素和依维莫司还抑制了p-mTOR和p-P70S6K的表达，并增加了PTEN和p-4EBP-1的表达。值得注意的是，单独使用芒柄花黄素可抑制p-Akt表达，但依维莫司则无此作用。
芒柄花黄素通过抑制Akt活性增强了依维莫司的杀瘤效果。
