用于抗菌和生物应用的硼氮掺杂聚羟基脂肪酸酯/壳聚糖纳米复合材料
Boron Nitride Doped Polyhydroxyalkanoate/Chitosan Nanocomposite for Antibacterial and Biological Applications.
作者信息
Mukheem Abdul, Shahabuddin Syed, Akbar Noor, Miskon Azizi, Muhamad Sarih Norazilawati, Sudesh Kumar, Ahmed Khan Naveed, Saidur Rahman, Sridewi Nanthini
机构信息
Department of Maritime Science and Technology, Faculty of Defence Science and Technology, National Defence University of Malaysia, Kuala Lumpur 57000, Malaysia.
Research Centre for Nano-Materials and Energy Technology (RCNMET), School of Science and Technology, Sunway University, Subang Jaya 47500, Malaysia.
出版信息
Nanomaterials (Basel). 2019 Apr 21;9(4):645. doi: 10.3390/nano9040645.
The present research focused on the fabrication of biocompatible polyhydroxyalkanoate, chitosan, and hexagonal boron nitride incorporated (PHA/Ch-hBN) nanocomposites through a simple solvent casting technique. The fabricated nanocomposites were comprehensively characterized by Fourier transform infrared spectroscope (FT-IR), field emission scanning electroscope (FESEM), and elemental mapping and thermogravimetric analysis (TGA). The antibacterial activity of nanocomposites were investigated through time-kill method against multi drug resistant (MDR) microbes such as methicillin-resistant (MRSA) and () K1 strains. In addition, nanocomposites have examined for their host cytotoxicity abilities using a Lactate dehydrogenase (LDH) assay against spontaneously immortalized human keratinocytes (HaCaT) cell lines. The results demonstrated highly significant antibacterial activity against MDR organisms and also significant cell viability as compared to the positive control. The fabricated PHA/Ch-hBN nanocomposite demonstrated effective antimicrobial and biocompatibility properties that would feasibly suit antibacterial and biomedical applications.
本研究聚焦于通过简单的溶液浇铸技术制备生物相容性聚羟基脂肪酸酯、壳聚糖和六方氮化硼复合（PHA/Ch-hBN）纳米复合材料。通过傅里叶变换红外光谱仪（FT-IR）、场发射扫描电子显微镜（FESEM）、元素映射和热重分析（TGA）对制备的纳米复合材料进行了全面表征。通过时间杀灭法研究了纳米复合材料对耐多药（MDR）微生物如耐甲氧西林金黄色葡萄球菌（MRSA）和（）K1菌株的抗菌活性。此外，使用乳酸脱氢酶（LDH）测定法针对自发永生化的人角质形成细胞（HaCaT）细胞系检测了纳米复合材料的宿主细胞毒性能力。结果表明，与阳性对照相比，该纳米复合材料对耐多药微生物具有高度显著的抗菌活性，且细胞活力也显著。制备的PHA/Ch-hBN纳米复合材料表现出有效的抗菌和生物相容性特性，有望适用于抗菌和生物医学应用。
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