School of Life Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, USA.
School of Life Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, USA
J Bacteriol. 2019 Aug 22;201(18). doi: 10.1128/JB.00050-19. Print 2019 Sep 15.
The ability of to form biofilms, which are communities of cells encased in a self-produced extracellular matrix, protects the cells from antibiotics and the host immune response. While some biofilm matrix components, such as exopolysaccharides and extracellular DNA, are relatively well characterized, the extracellular matrix proteins remain understudied. Multiple proteomic analyses of the soluble biofilm matrix and outer membrane vesicles, which are a component of the matrix, have identified OprF as an abundant matrix protein. To date, the few reports on the effects of mutations on biofilm formation are conflicting, and little is known about the potential role of OprF in the biofilm matrix. The majority of OprF studies focus on the protein as a cell-associated porin. As a component of the outer membrane, OprF assumes dual conformations and is involved in solute transport, as well as cell envelope integrity. Here, we review the current literature on OprF in , discussing how the structure and function of the cell-associated and matrix-associated protein may affect biofilm formation and pathogenesis in order to inform future research on this understudied matrix protein.
能够形成生物膜，生物膜是由细胞包裹在自身产生的细胞外基质中的群落，这使细胞能够免受抗生素和宿主免疫反应的影响。虽然一些生物膜基质成分，如胞外多糖和胞外 DNA，已经得到了相对较好的描述，但细胞外基质蛋白仍然研究不足。对 可溶性生物膜基质和外膜囊泡（基质的一个组成部分）的多次蛋白质组学分析已经确定 OprF 是一种丰富的基质蛋白。迄今为止，关于 突变对生物膜形成影响的少数报告相互矛盾，并且对 OprF 在生物膜基质中的潜在作用知之甚少。大多数关于 OprF 的研究都集中在该蛋白作为细胞相关孔蛋白上。作为外膜的一部分，OprF 呈现出两种构象，参与溶质运输以及细胞包膜的完整性。在这里，我们回顾了关于 OprF 在 中的现有文献，讨论了细胞相关和基质相关蛋白的结构和功能如何影响生物膜形成和发病机制，以便为这一研究不足的基质蛋白的未来研究提供信息。
