Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Biol Trace Elem Res. 2020 Feb;193(2):410-413. doi: 10.1007/s12011-019-01728-0. Epub 2019 Apr 26.
This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO strongly inhibited the replication of virus progeny (MOI 0.001) at least 68-fold less. On the other hand, the IC50 demonstrated that the highest activity of ZnSO was at the 0.23 mM concentration.
本报告解释了采用传统细胞培养与实时定量 PCR 相结合的方法评估硫酸锌（ZnSO）对单纯疱疹病毒（HSV）感染的 Vero 细胞的抗病毒作用。我们的证据表明，用 0.3 mM ZnSO 处理可至少抑制病毒后代的复制 68 倍（MOI 0.001）。另一方面，IC50 表明 ZnSO 的最高活性在 0.23 mM 浓度下。
