Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan.
Department of Stomatology, MacKay Memorial Hospital, Taipei, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:630-639. doi: 10.1016/j.msec.2019.04.018. Epub 2019 Apr 9.
A novel multifunctional poly(γ-glutamic acid) (γ-PGA)/gelatin hydrogel has been developed and used as a wound dressing. An ideal wound dressing should effectively provide a moist environment, absorb wound exudates and protect the wound from foreign microbes. Water soluble γ-PGA salts of sodium and calcium forms were chosen for their good biocompatibility, biodegradability and water absorption capacity. Oligomeric proanthocyanidins (OPCs), naturally occurring plant metabolites and potent antioxidants, were investigated as a non-toxic crosslinking agent in this study. The effects of hydrogels on the degree of crosslinking, swelling, in vitro degradation, mechanical properties and radical scavenging activity were systemically evaluated. A cell viability assay demonstrated that these OPCs crosslinked γ-PGA/gelatin (PGO) hydrogels were not cytotoxic to L929 fibroblasts. Dermal irritation and skin sensitization tests were examined using a guinea pig model; the hydrogels were considered to be neither allergic nor a dermal sensitizer in guinea pigs. Lastly, an in vivo wound healing model in rats was used to study the effects of the hydrogels on wound healing for 21 days. PGO hydrogels formed by both Na and Ca salts could accelerate wound contraction and re-epithelialization, in which Na-PGO hydrogel was significantly better than the untreated control group. The findings suggest that PGO hydrogels are promising wound dressing materials for the treatment for wound healing.
一种新型多功能聚（γ-谷氨酸）（γ-PGA）/明胶水凝胶已被开发并用作伤口敷料。理想的伤口敷料应能有效地提供湿润的环境，吸收伤口渗出物，并防止伤口受到外来微生物的侵害。选择水溶性的γ-PGA 钠盐和钙盐，因其具有良好的生物相容性、可生物降解性和吸水性。本研究将低聚原花青素（OPCs），一种天然存在的植物代谢物和有效的抗氧化剂，作为一种无毒的交联剂进行了研究。系统评估了水凝胶对交联度、溶胀、体外降解、机械性能和自由基清除活性的影响。细胞活力测定表明，这些 OPCs 交联的 γ-PGA/明胶（PGO）水凝胶对 L929 成纤维细胞没有细胞毒性。通过豚鼠模型对皮肤刺激性和皮肤致敏性进行了测试；水凝胶在豚鼠中既不过敏也不引起皮肤致敏。最后，使用大鼠体内伤口愈合模型研究了水凝胶对 21 天伤口愈合的影响。Na 和 Ca 盐形成的 PGO 水凝胶可以加速伤口收缩和再上皮化，其中 Na-PGO 水凝胶明显优于未处理的对照组。研究结果表明，PGO 水凝胶是一种有前途的伤口敷料材料，可用于治疗伤口愈合。
