在野外条件下使用环己酰亚胺处理的细胞分离沙眼病原体。
The use of cycloheximide-treated cells for isolating trachoma agents under field conditions.
作者信息
Schachter J, Dawson C R, Hoshiwara I, Daghfous T, Banks J
出版信息
Bull World Health Organ. 1978;56(4):629-32.
Abstract
Standard procedures for the isolation of Chlamydia trachomatis require pretreatment of the tissue culture cells. We evaluated the use of cycloheximide, an antimetabolite that can be added to the cells with the inoculum. Cycloheximide-treated cells provided a sensitive system for isolating trachoma agents. This system was applicable to field studies as requirements for equipment were minimal and the cells were 2 weeks old when inoculated.
摘要
沙眼衣原体分离的标准程序需要对组织培养细胞进行预处理。我们评估了放线菌酮的使用，放线菌酮是一种抗代谢物，可以与接种物一起添加到细胞中。经放线菌酮处理的细胞为沙眼病原体的分离提供了一个敏感系统。该系统适用于现场研究，因为所需设备最少，且接种时细胞已有两周龄。
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本文引用的文献
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ISOLATION OF THE TRACHOMA AGENT IN CELL CULTURE.在细胞培养中分离沙眼病原体。
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Effect of infection with the meningopneumonitis agent on deoxyribonucleic acid and protein synthesis by its L-cell host.脑膜炎肺炎病原体感染对其L细胞宿主的脱氧核糖核酸和蛋白质合成的影响。
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Chlamydial infection. Advances in the diagnostic isolation of Chlamydia, including TRIC agent, from the eye, genital tract, and rectum.衣原体感染。从眼睛、生殖道和直肠诊断分离包括沙眼包涵体结膜炎病原体在内的衣原体方面的进展。
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Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells.使用经5-碘-2-脱氧尿苷处理的细胞分离沙眼衣原体。
Appl Microbiol. 1974 May;27(5):912-6. doi: 10.1128/am.27.5.912-916.1974.
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Severe endemic trachoma in tunisia. II. A controlled therapy trial of topically applied chlortetracycline and erythromycin.突尼斯的严重地方性沙眼。II. 局部应用金霉素和红霉素的对照治疗试验。
Arch Ophthalmol. 1974 Sep;92(3):198-203. doi: 10.1001/archopht.1974.01010010206003.
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Severe endemic trachoma in Tunisia.突尼斯的严重地方性沙眼
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