Gibbons R J, Qureshi J V
Infect Immun. 1978 Dec;22(3):665-71. doi: 10.1128/iai.22.3.665-671.1978.
Strains of Streptococcus mutans of four genetic groups and five serotypes, and strains of S. sanguis, S. mitis, S. salivarius, Actinomyces naeslundii, and A. viscosus, were found to bind blood group-reactive (BGR) mucin isolated from whole human saliva. The bacteria studied bound mucins with blood type A or B reactivity to a similar extent, suggesting that the carbohydrate moieties responsible for the A and B antigenic determinants were not involved. The organisms studied appeared to bind different fractions of BGR mucin molecules because preparations absorbed with cells of a given oral species no longer contained BGR molecules which bound to homologous organisms but still possessed BGR components which bound to varying degrees to other bacteria. Differences were even noted among S. mutans strains belonging to different genetic groups and serological types. Immunoglobulins could not be detected in the mucin preparations, and addition of anti-human immunoglobulin A (IgA), IgG, or IgM serum to reaction mixtures did not affect binding. Mucin pretreated with periodate or iodoacetate no longer bound to S. mutans H12 cells, suggesting that carbohydrate moieties and sulfhydryl groups played an essential role. Active cell metabolism was not required for BGR mucin binding; however, pretreatment of H12 cells with periodate or heat (100 degrees C for 15 min) reduced binding. Mucin labeled with [(14)C]phenyl isothiocyanate appeared to bind to S. mutans H12 cells comparably to untreated mucin; the binding also appeared to be specific because less than 15% of the labeled material became bound when incubated with an excess of streptococci. Binding of [(14)C]phenyl isothiocyanate-labeled mucin was not affected by neutral sugars tested or by preparations of c antigen, glycerol teichoic acid, dextran, or crude glucosyltransferase. However, binding was inhibited by several amines. BGR salivary mucins are present in the acquired pellicle covering teeth; the ability of bacteria to selectively bind such components suggest that they may serve as receptor molecules involved in the attachment of bacteria to teeth.
研究发现，四个遗传组和五个血清型的变形链球菌菌株，以及 sanguis 链球菌、缓症链球菌、唾液链球菌、内氏放线菌和黏性放线菌的菌株，能够结合从全人类唾液中分离出的血型反应性（BGR）黏蛋白。所研究的细菌对具有 A 型或 B 型反应性的黏蛋白的结合程度相似，这表明负责 A 和 B 抗原决定簇的碳水化合物部分并未参与其中。所研究的生物体似乎结合了 BGR 黏蛋白分子的不同部分，因为用特定口腔物种的细胞吸收后的制剂不再含有与同源生物体结合的 BGR 分子，但仍具有与其他细菌不同程度结合的 BGR 成分。甚至在属于不同遗传组和血清型的变形链球菌菌株之间也观察到了差异。在黏蛋白制剂中未检测到免疫球蛋白，并且向反应混合物中添加抗人免疫球蛋白 A（IgA）、IgG 或 IgM 血清不会影响结合。用过碘酸盐或碘乙酸预处理的黏蛋白不再与变形链球菌 H12 细胞结合，这表明碳水化合物部分和巯基起着至关重要的作用。BGR 黏蛋白结合不需要活跃的细胞代谢；然而，用过碘酸盐或加热（100℃ 15 分钟）预处理 H12 细胞会降低结合。用 [(14)C]苯基异硫氰酸酯标记的黏蛋白与未处理的黏蛋白相比，似乎能与变形链球菌 H12 细胞结合；这种结合似乎也是特异性的，因为与过量链球菌一起孵育时，不到 15%的标记物质会结合。[(十四)C]苯基异硫氰酸酯标记的黏蛋白结合不受所测试的中性糖或 c 抗原、甘油磷壁酸、葡聚糖或粗制葡糖基转移酶制剂的影响。然而，几种胺会抑制结合。BGR 唾液黏蛋白存在于覆盖牙齿的获得性膜中；细菌选择性结合此类成分的能力表明它们可能作为参与细菌附着于牙齿的受体分子。
