Mayner R E, Needy C F
J Biol Stand. 1987 Jan;15(1):1-10. doi: 10.1016/0092-1157(87)90011-4.
The glycoprotein content of rabies vaccines containing the Pitman-Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6.3% for a single vaccine lot tested over a one-year period. Using sera prepared against rabies virus glycoprotein from different strains of virus, the assay gave different values. These differences could be eliminated by using a homologous vaccine strain as an internal reference. Single radial-immunodiffusion values for Pitman-Moore vaccines correlated with the manufacturers' NIH potency assay, but required a mathematical transformation to convert values from one assay to the other. Single radial-immunodiffusion values for Street Alabama Dufferin and Flury-LEP vaccines did not correlate with NIH values. Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed.
采用单向辐射免疫扩散试验测定了含狂犬病病毒皮特曼 - 摩尔株的狂犬病疫苗的糖蛋白含量，并将其与疫苗效力相关联。在一年时间内对单个疫苗批次进行测试，该试验的变异性为6.3%。使用针对不同病毒株的狂犬病病毒糖蛋白制备的血清进行检测时，该试验得出了不同的值。通过使用同源疫苗株作为内部参考，可以消除这些差异。皮特曼 - 摩尔疫苗的单向辐射免疫扩散值与制造商的国立卫生研究院（NIH）效力测定相关，但需要进行数学转换才能将一个试验的值转换为另一个试验的值。阿拉巴马街达弗林毒株和弗勒里 - LEP疫苗的单向辐射免疫扩散值与NIH值不相关。本文讨论了单向辐射免疫扩散技术的改进以及使用该试验测定狂犬病疫苗效力的可行性。
