a Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry , Nankai University , Tianjin , China.
J Biomater Sci Polym Ed. 2019 Jul;30(10):815-831. doi: 10.1080/09205063.2019.1603065. Epub 2019 May 2.
We reported on the fabrication of sugar-responsive nanogels covalently incorporated with 3-acrylamidophenylboronic acid (AAPBA) as glucose-recognizing moiety, 2-(acrylamido)glucopyranose (AGA) as biocompatible moiety, and boron dipyrromethene (BODIPYMA) as fluorescence donor molecule. The p(AAPBA-AGA-BODIPYMA) nanogels were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization in the mixture solvents of HO/ethanol. Nanogels could respond to glucose and size of nanogels increased after treating with 3 mg/mL glucose medium. The fluorescent intensity of nanogels varied dependent on different glucose concentrations. Besides, insulin, a model drug, can be encapsulated into nanogels with the loading amount up to 8.2%. The drug release was dependent on the content of AAPBA moieties in nanogels and glucose concentrations in release medium. The investigation on the cytotoxicity of nanogels revealed that nanogels had good compatibility. Such glucose-responsive nanogels have potential in detection and treatment of diabetes.
我们报道了糖响应纳米凝胶的制备，该纳米凝胶通过可逆加成-断裂链转移（RAFT）聚合在 HO/乙醇混合溶剂中合成，其中含有作为葡萄糖识别部分的 3-丙烯酰胺基苯硼酸（AAPBA）、作为生物相容性部分的 2-（丙烯酰胺基）吡喃葡萄糖（AGA）和作为荧光供体分子的硼二吡咯甲川（BODIPYMA）。纳米凝胶可以响应葡萄糖，并且在 3mg/mL 葡萄糖介质处理后，纳米凝胶的尺寸增加。纳米凝胶的荧光强度随葡萄糖浓度的不同而变化。此外，胰岛素作为模型药物可以被包封到纳米凝胶中，载药量高达 8.2%。药物释放取决于纳米凝胶中 AAPBA 部分的含量和释放介质中的葡萄糖浓度。对纳米凝胶细胞毒性的研究表明，纳米凝胶具有良好的相容性。这种葡萄糖响应纳米凝胶在糖尿病的检测和治疗方面具有潜在的应用价值。
