乳酸克鲁维酵母β-半乳糖苷酶基因的正调控由一个上游激活位点介导，该位点与酿酒酵母的GAL上游激活位点具有同源性。
Positive regulation of the beta-galactosidase gene from Kluyveromyces lactis is mediated by an upstream activation site that shows homology to the GAL upstream activation site of Saccharomyces cerevisiae.
作者信息
Ruzzi M, Breunig K D, Ficca A G, Hollenberg C P
出版信息
Mol Cell Biol. 1987 Mar;7(3):991-7. doi: 10.1128/mcb.7.3.991-997.1987.
Abstract
In contrast to the Escherichia coli lac operon, the yeast beta-galactosidase gene is positively regulated. In the 5'-noncoding region of the Kluyveromyces lactis LAC4 gene, we mapped an upstream activation site (UAS) that is required for induction. This sequence, located between positions -435 and -326 from the start of translation, functions irrespective of its orientation and can confer lactose regulation to the heterologous CYC1 promoter. It is composed of at least two subsequences that must act in concert. One of these subsequences showed a strong homology to the UAS consensus sequence of the Saccharomyces cerevisiae GAL genes (E. Giniger, S. M. Varnum, and M. Ptashne, Cell 40:767-774, 1985). We propose that this region of homology located at about position -426 is a binding site for the product of the regulatory gene LAC9 which probably induces transcription of the LAC4 gene in a manner analogous to that of the GAL4 protein.
摘要
与大肠杆菌乳糖操纵子不同，酵母β-半乳糖苷酶基因受到正调控。在乳酸克鲁维酵母LAC4基因的5'-非编码区，我们定位了一个诱导所需的上游激活位点（UAS）。该序列位于翻译起始点-435至-326之间，无论其方向如何都能发挥作用，并且可以赋予异源CYC1启动子乳糖调节功能。它由至少两个必须协同作用的子序列组成。这些子序列之一与酿酒酵母GAL基因的UAS共有序列具有很强的同源性（E. Giniger、S. M. Varnum和M. Ptashne，《细胞》40:767 - 774，1985年）。我们推测位于约-426位置的这个同源区域是调控基因LAC9产物的结合位点，LAC9可能以类似于GAL4蛋白的方式诱导LAC4基因的转录。
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Positive regulation of the beta-galactosidase gene from Kluyveromyces lactis is mediated by an upstream activation site that shows homology to the GAL upstream activation site of Saccharomyces cerevisiae.乳酸克鲁维酵母β-半乳糖苷酶基因的正调控由一个上游激活位点介导，该位点与酿酒酵母的GAL上游激活位点具有同源性。
Mol Cell Biol. 1987 Mar;7(3):991-7. doi: 10.1128/mcb.7.3.991-997.1987.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Lac4 is the structural gene for beta-galactosidase in Kluyveromyces lactis.Lac4是乳酸克鲁维酵母中β-半乳糖苷酶的结构基因。
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