Pedersen N S, Kam-Hansen S, Link H, Mavra M
Acta Pathol Microbiol Immunol Scand C. 1982 Apr;90(2):97-104. doi: 10.1111/j.1699-0463.1982.tb01424.x.
Cerebrospinal fluid from four patients with serologically and clinically verified neurosyphilis contained oligoclonal bands when analysed by isoelectric focusing. By antiserum immunofixation these bands consisted by IgG, and by antigen immunofixation and autoradiography the bands were shown to contain treponemal antibodies. Treponemal antibodies synthesized within the CNS were also detected in polyclonal CSF IgG fractions in two of the patients. In one patient with neurosyphilis followed over 2.5 years, local synthesis of IgM and IgA disappeared within 1 year after treatment, while IgG synthesis persisted. In this patient, besides treponemal antibodies, antibodies against herpes simplex virus were also detected in oligoclonal and polyclonal IgG fractions of CSF. The presence of treponemal antibodies synthesized within the CNS was identified in 2 of 16 controls with negative serological tests for syphilis. This might reflect a previous contact with apathogenic treponemes, and the intrathecal antibody synthesis might be a result of an unspecific activation of memory lymphocytes in CNS.
通过等电聚焦分析，4例经血清学和临床确诊为神经梅毒患者的脑脊液中含有寡克隆带。通过抗血清免疫固定法，这些条带由IgG组成，通过抗原免疫固定法和放射自显影法显示这些条带含有梅毒螺旋体抗体。在2例患者的多克隆脑脊液IgG组分中也检测到中枢神经系统内合成的梅毒螺旋体抗体。在1例随访超过2.5年的神经梅毒患者中，治疗后1年内局部IgM和IgA合成消失，而IgG合成持续存在。在该患者中，除了梅毒螺旋体抗体外，在脑脊液的寡克隆和多克隆IgG组分中还检测到抗单纯疱疹病毒抗体。在16例梅毒血清学检测阴性的对照中有2例检测到中枢神经系统内合成的梅毒螺旋体抗体。这可能反映了既往与致病性梅毒螺旋体的接触，鞘内抗体合成可能是中枢神经系统中记忆淋巴细胞非特异性激活的结果。
