College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China.
Food Funct. 2019 May 22;10(5):2805-2816. doi: 10.1039/c9fo00074g.
Egg phosvitin has been traditionally considered as a nutritionally negative protein for its low digestibility in the gastrointestinal tract. Whether it could exert nutritional functions in the large intestine is not known. In this work, the influence of phosvitin on luminal microbiota composition and mucosal transcriptome was investigated with young (3-week) and adult (8-week) mouse models. In young groups, phosvitin mainly suppressed genes related to lipid metabolism, whereas the regulated genes in adult individuals encompassed various biological processes, such as carbohydrate metabolism, sigestive system and others. Phosvitin increased the proportion of Bifidobacterium in the young group, and reduced amounts of pathogenic microbes in the adult group, including Helicobacter and Mucispirillum. There was a close relationship between gene expression changes and abundance of bacteria. Finally, phosvitin reduced the ammonia concentrations in feces for both young and adult groups. These findings suggested that phosvitin modified the large intestinal ecosystem, exhibiting potentially beneficial effects on gut health.
卵黄高磷蛋白传统上被认为是一种在胃肠道中消化率低的营养负性蛋白。然而，它是否能在大肠中发挥营养功能尚不清楚。在这项工作中，使用年轻（3 周）和成年（8 周）小鼠模型研究了卵黄高磷蛋白对腔微生物组成和粘膜转录组的影响。在年轻组中，卵黄高磷蛋白主要抑制与脂质代谢相关的基因，而成年个体中受调控的基因则涵盖了各种生物学过程，如碳水化合物代谢、消化系统等。卵黄高磷蛋白增加了双歧杆菌在年轻组中的比例，并减少了成年组中包括幽门螺旋杆菌和黏液螺旋菌在内的致病性微生物的数量。基因表达变化与细菌丰度之间存在密切关系。最后，卵黄高磷蛋白降低了年轻和成年组粪便中的氨浓度。这些发现表明，卵黄高磷蛋白改变了大肠生态系统，对肠道健康可能具有有益的影响。
