Department of Biotechnology, Institute of Agrochemistry and Food Technology-National Research Council (IATA-CSIC), Av. Agustin Escardino 7, 46980, Valencia, Spain.
Health care center La Eliana, Valencia, Spain.
BMC Pediatr. 2019 May 3;19(1):140. doi: 10.1186/s12887-019-1502-y.
Early microbial colonization is a relevant aspect in human health. Altered microbial colonization patterns have been linked to an increased risk of non-communicable diseases (NCDs). Advances in understanding host-microbe interactions highlight the pivotal role of maternal microbiota on infant health programming. This birth cohort is aimed to characterize the maternal microbes transferred to neonates during the first 1000 days of life, as well as to identify the potential host and environmental factors, such as gestational age, mode of delivery, maternal/infant diet, and exposure to antibiotics, which affect early microbial colonization.
MAMI is a prospective mother-infant birth cohort in the Spanish-Mediterranean area. Mothers were enrolled at the end of pregnancy and families were follow-up during the first years of life. Maternal-infant biological samples were collected at several time points from birth to 24 months of life. Clinical and anthropometric characteristics and dietary information is available. Specific qPCR and 16S rRNA gene sequencing as well as short chain fatty acid (SCFAs) profile would be obtained. Multivariable models will be used to identy associations between microbiota and clinical and anthropometric data controlling for confounders. MAMI would contribute to a better understanding of the interaction between diet, microbiota and host response in early life health programming, enabling new applications in the field of personalized nutrition and medicine.
The study is registered on the ClinicalTrial.gov platform NCT03552939. (June 12, 2018).
早期微生物定植是人类健康的一个重要方面。微生物定植模式的改变与非传染性疾病（NCD）的风险增加有关。对宿主-微生物相互作用的深入了解强调了母体微生物对婴儿健康发育的关键作用。本队列旨在描述生命最初 1000 天内母体微生物向新生儿的转移，并确定潜在的宿主和环境因素，如胎龄、分娩方式、母婴饮食和抗生素暴露，这些因素影响早期微生物定植。
MAMI 是西班牙-地中海地区的一项前瞻性母婴出生队列研究。母亲在妊娠末期入组，在婴儿生命的最初几年进行随访。从出生到 24 个月的生命期间，在多个时间点采集母婴生物样本。可获得临床和人体测量特征以及饮食信息。将进行特定的 qPCR 和 16S rRNA 基因测序以及短链脂肪酸（SCFA）谱分析。将使用多变量模型来识别微生物群与临床和人体测量数据之间的关联，同时控制混杂因素。MAMI 将有助于更好地理解饮食、微生物群和宿主对早期生命健康发育的相互作用，为个性化营养和医学领域的新应用提供支持。
该研究在 ClinicalTrial.gov 平台上注册，注册号为 NCT03552939。（2018 年 6 月 12 日）。
