Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran.
Cytokine. 2019 Aug;120:99-106. doi: 10.1016/j.cyto.2019.04.012. Epub 2019 May 1.
The role of myostatin (MSTN) in the regulation of energy homeostasis has been known and that MSTN inhibition can attenuate the development of diabetes. However, the response of MSTN to exercise in type 1 diabetes (T1DM) is unknown. This study aimed to investigate the alteration of MSTN following aerobic exercise training in diabetic rats and its possible interaction with glycosylated hemoglobin (HbA1c) and inflammatory cytokines. Forty-eight male Wistar rats were divided into non-diabetic untrained, non-diabetic trained, diabetic untrained and diabetic trained groups. To induce T1DM, rats received an intraperitoneal injection of STZ (60 mg·kg). Treadmill exercise was performed for six weeks, five days/week. HbA1c was estimated, MSTN mRNA expression in skeletal muscle was measured, and plasma MSTN and inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) were determined. Results revealed a significant decrease of HbA1c and plasma inflammatory cytokines (IL-6, TNF-α and IL-1β) followed by a significant decrease of plasma and skeletal muscle MSTN in diabetic trained rats versus non-diabetic untrained and diabetic untrained rats after the experimental period. Moreover, in diabetic untrained and diabetic trained rats, a significantly positive correlation (change versus change) of plasma MSTN with HbA1c and plasma IL-6, TNF-α and IL-1β was found. In conclusion, this study indicated that aerobic exercise training by a decrease of HbA1c and plasma IL-6, TNF-α and IL-1β could decrease MSTN levels in plasma and skeletal muscle in T1DM. Furthermore, the effective influence of exercise may be reflected by changes of MSTN in diabetic rats.
肌肉生长抑制素 (MSTN) 在能量稳态调节中的作用已经为人所知，并且 MSTN 抑制可以减轻糖尿病的发展。然而，1 型糖尿病 (T1DM) 中 MSTN 对运动的反应尚不清楚。本研究旨在探讨有氧运动训练后糖尿病大鼠 MSTN 的变化及其与糖化血红蛋白 (HbA1c) 和炎症细胞因子的可能相互作用。48 只雄性 Wistar 大鼠分为非糖尿病未训练组、非糖尿病训练组、糖尿病未训练组和糖尿病训练组。为了诱导 T1DM，大鼠接受 STZ（60mg·kg）腹腔注射。进行六周的跑步机运动，每周五天。估计 HbA1c，测量骨骼肌 MSTN mRNA 表达，并测定血浆 MSTN 和炎症细胞因子，包括白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α) 和白细胞介素-1β (IL-1β)。结果显示，与非糖尿病未训练组和糖尿病未训练组相比，糖尿病训练组在实验期间 HbA1c 和血浆炎症细胞因子（IL-6、TNF-α 和 IL-1β）显著降低，随后血浆和骨骼肌 MSTN 显著降低。此外，在糖尿病未训练和糖尿病训练大鼠中，发现血浆 MSTN 与 HbA1c 和血浆 IL-6、TNF-α 和 IL-1β 的变化呈显著正相关。总之，本研究表明，有氧运动训练通过降低 HbA1c 和血浆 IL-6、TNF-α 和 IL-1β 可以降低 T1DM 大鼠血浆和骨骼肌中的 MSTN 水平。此外，运动的有效影响可能反映在糖尿病大鼠 MSTN 的变化上。
