运动训练可降低Zucker糖尿病（2型）肥胖大鼠的促炎状态。

Exercise training decreases proinflammatory profile in Zucker diabetic (type 2) fatty rats.

作者信息

Teixeira de Lemos E, Reis F, Baptista S, Pinto R, Sepodes B, Vala H, Rocha-Pereira P, Correia da Silva G, Teixeira N, Silva A Santos, Carvalho L, Teixeira F, Das U N

机构信息

Therapeutics Unit, Institute of Pharmacology and Experimental Therapeutics, Medicine Faculty, Coimbra University, Coimbra, Portugal; Polytechnic Institute of Viseu, Viseu, Portugal.

出版信息

Nutrition. 2009 Mar;25(3):330-9. doi: 10.1016/j.nut.2008.08.014. Epub 2008 Dec 5.

DOI:10.1016/j.nut.2008.08.014

PMID:19062255

Abstract

OBJECTIVE

In the present study we evaluated the effect of exercise on the plasma levels of proinflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), and the anti-inflammatory molecule uric acid in the Zucker diabetic fatty (ZDF) rats that are more prone to develop type 2 diabetes mellitus.

METHODS

Sixteen obese ZDF (Gmi fa/fa) rats (8 wk old, 228.40 +/- 4.05 g) were randomly assigned to one of two groups (n = 8 each): an exercise-trained group and a sedentary one. In addition, 16 lean ZDF (Gmi +/+) rats (8 wk old, 199.00 +/- 3.50 g) were subjected to identical sedentary and exercise conditioning (n = 8 each). Initially, rats swam 15 min/d (5 d/wk) in a 36 degrees C bath. The exercise protocol was gradually increased by 15 min/d until a swimming period of 1 h/d (1 wk) was attained. Thereafter, rats swam 1 h/d, 3 d/wk, for an additional period of 11 wk. Rats were sacrificed 48 h after the last training period and the blood and pancreas were collected. Circulating levels of glucose, glycosylated hemoglobin, total cholesterol, triglycerides, insulin, uric acid, IL-6, and TNF-alpha were assessed. The concentrations of proinflammatory cytokines in the pancreas were also evaluated.

RESULTS

In the diabetic ZDF (fa/fa) rats, exercise decreased hyperuricemia (-37.3%) and IL-6 and TNF-alpha levels (-16.9% and -12.7% respectively) and maintained the weight of the pancreas at near normal. Immunohistochemistry revealed a marked decrease in the expression of TNF-alpha and IL-6 in the pancreatic islet cells of ZDF (fa/fa) rats.

CONCLUSION

These results indicate that aerobic exercise is anti-inflammatory in nature.

摘要

目的

在本研究中，我们评估了运动对更易患2型糖尿病的Zucker糖尿病脂肪（ZDF）大鼠血浆促炎细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平以及抗炎分子尿酸的影响。

方法

16只肥胖ZDF（Gmi fa/fa）大鼠（8周龄，228.40±4.05 g）被随机分为两组（每组n = 8）：运动训练组和久坐组。此外，16只瘦ZDF（Gmi +/+）大鼠（8周龄，199.00±3.50 g）接受相同的久坐和运动条件（每组n = 8）。最初，大鼠在36℃水浴中每天游泳15分钟（每周5天）。运动方案每天逐渐增加15分钟，直至达到每天游泳1小时（1周）。此后，大鼠每天游泳1小时，每周3天，持续11周。在最后一个训练期后48小时处死大鼠，收集血液和胰腺。评估循环中的葡萄糖、糖化血红蛋白、总胆固醇、甘油三酯、胰岛素、尿酸、IL-6和TNF-α水平。还评估了胰腺中促炎细胞因子的浓度。