Ateya Ahmed I, Arafat Nagah, Saleh Rasha M, Ghanem Hanaa M, Naguib Doaa, Radwan Hend A, Elseady Y Y
Department of Animal Husbandry and Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Vet Res Commun. 2019 May;43(2):131-142. doi: 10.1007/s11259-019-09753-z. Epub 2019 May 4.
In this study, we investigated the effects of probiotic, acidifier and synbiotic supplementation on growth performance, mortality rate, intestinal gene expressions, fecal shedding, and organs colonization induced by Escherichia coli in broiler chickens. Six experimental groups were included; negative control group (NC), positive control group (PC), probiotic group (PR), acidifier group (AC), synbiotic group (SY) and colistin sulfate group (CS). Chickens in groups NC and PC were fed a basal diet, while chickens in groups PR, AC, SY, and CS were fed a basal diet containing probiotic, acidifier, synbiotic and colistin sulfate, respectively from the 1st day to the 28th day of age. At 7 days of age, all groups (not NC) were orally challenged with 0.5 ml (1.0 × 10 CFU/ml) E. coli O78. The dietary supplementation of acidifier and synbiotic were sufficient to quell the devastating effects of E. coli infection in broilers. Growth performances represented by body weight gain, feed intake and feed conversion ratio were significantly improved as well as, mortalities were prevented whilst the ileal pro-inflammatory gene expressions (IL-6, IL-8, IL-13, TLR-4, IFN-γ, LITAF, AvBD-2, and AvBD-9) were significantly downregulated and the anti-inflammatory cytokine (IL-10) was significantly increased. In addition, E. coli fecal shedding and organs colonization was significantly diminished. It was concluded that the addition of both acidifier and synbiotic to the diet of broilers infected with E. coli could modulate the intestinal inflammatory responses induced by E. coli infection and minimized the inflammation-induced damage which resulted in improvement in growth performance, prevention of mortalities and reduction of E. coli environmental contamination.
在本研究中，我们调查了补充益生菌、酸化剂和合生元对肉鸡生长性能、死亡率、肠道基因表达、粪便排菌以及大肠杆菌诱导的器官定植的影响。实验分为六个组，即阴性对照组（NC）、阳性对照组（PC）、益生菌组（PR）、酸化剂组（AC）、合生元组（SY）和硫酸黏菌素组（CS）。NC组和PC组的鸡饲喂基础日粮，而PR组、AC组、SY组和CS组的鸡从1日龄至28日龄分别饲喂添加了益生菌、酸化剂、合生元和硫酸黏菌素的基础日粮。7日龄时，所有组（不包括NC组）口服接种0.5毫升（1.0×10⁸CFU/毫升）大肠杆菌O78。日粮中添加酸化剂和合生元足以减轻大肠杆菌感染对肉鸡的破坏性影响。以体重增加、采食量和饲料转化率表示的生长性能显著提高，同时死亡率得到预防，回肠促炎基因表达（IL-6、IL-8、IL-13、TLR-4、IFN-γ、LITAF、AvBD-2和AvBD-9）显著下调，抗炎细胞因子（IL-10）显著增加。此外，大肠杆菌粪便排菌和器官定植显著减少。得出的结论是，在感染大肠杆菌的肉鸡日粮中添加酸化剂和合生元可调节大肠杆菌感染诱导的肠道炎症反应，并将炎症引起的损害降至最低，从而改善生长性能、预防死亡并减少大肠杆菌对环境的污染。
