Figueroa Benjamin, Fu Walter, Nguyen Tai, Shin Kseniya, Manifold Bryce, Wise Frank, Fu Dan
Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
Biomed Opt Express. 2018 Nov 8;9(12):6116-6131. doi: 10.1364/BOE.9.006116. eCollection 2018 Dec 1.
Hyperspectral stimulated Raman scattering (hsSRS) microscopy has recently emerged as a powerful non-destructive technique for the label-free chemical imaging of biological samples. In most hsSRS imaging experiments, the SRS spectral range is limited by the total bandwidth of the excitation laser to ~300 cm and a spectral resolution of ~25 cm. Here we present a novel approach for broadband hsSRS microscopy based on parabolic fiber amplification to provide linearly chirped broadened Stokes pulses. This novel hsSRS instrument provides >600 cm spectral coverage and ~10 cm spectral resolution. We further demonstrated broadband hsSRS imaging of the entire Raman fingerprint region for resolving the distribution of major biomolecules in fixed cells. Moreover, we applied broadband hsSRS in imaging amyloid plaques in human brain tissue with Alzheimer's disease.
高光谱受激拉曼散射（hsSRS）显微镜最近已成为一种强大的非破坏性技术，用于对生物样品进行无标记化学成像。在大多数hsSRS成像实验中，SRS光谱范围受激发激光的总带宽限制，约为300 cm，光谱分辨率约为25 cm。在此，我们提出了一种基于抛物线光纤放大的宽带hsSRS显微镜新方法，以提供线性啁啾展宽的斯托克斯脉冲。这种新型hsSRS仪器提供了>600 cm的光谱覆盖范围和约10 cm的光谱分辨率。我们进一步展示了对整个拉曼指纹区域的宽带hsSRS成像，以解析固定细胞中主要生物分子的分布。此外，我们将宽带hsSRS应用于对患有阿尔茨海默病的人类脑组织中的淀粉样斑块进行成像。
