Department of Biological Sciences, Universidad de los Andes, Bogotá, 111711, Colombia.
Corporación Colombiana de Investigación Agropecuaria - Corpoica, Bogotá, Cundinamarca, 250047, Colombia.
Poult Sci. 2019 Oct 1;98(10):5054-5063. doi: 10.3382/ps/pez251.
According to the World Health Organization, Salmonella is one of the most important zoonotic foodborne pathogens. Poultry products are thought to be the main source of Salmonella, which means that it is necessary to control Salmonella at the pre-harvest stage. Bacteriophages, acting as host-specific parasites of bacterial cells, represent one of the alternatives to antibiotics that can contribute to food safety and security. The present study evaluated the effectiveness of the bacteriophage cocktail SalmoFREE® to control Salmonella on a commercial broiler farm. We assessed the relationship between the use of SalmoFREE® and productivity parameters (feed conversion, weight gain, homogeneity). Two field trials (trial 1 n = 34,986; trial 2 n = 34,680) were carried out under commercial rearing conditions on a Colombian broiler farm with a record of Salmonella presence. Each trial comprised 2 control chicken houses and 2 experimental ones. SalmoFREE® and a control suspension were delivered in the drinking water at 3 time points in the production cycle, and the presence of Salmonella was assessed in cloacal swabs the day before and after the treatments. Results revealed that SalmoFREE® controls the incidence of Salmonella and does not affect the animals nor the production parameters, demonstrating its efficacy and innocuity at the production scale. We detected phage-specific genes in samples of total DNA extracted from ceca after the treatment with SalmoFREE®, and tested for the appearance of cocktail-resistant Salmonella, which showed to be an uncommon event. These results contribute relevant information to the adoption of phage therapy as an alternative to growth-promoter antibiotics on poultry farms.
根据世界卫生组织的数据，沙门氏菌是最重要的动物源食源性致病菌之一。家禽产品被认为是沙门氏菌的主要来源，这意味着有必要在收获前阶段控制沙门氏菌。噬菌体作为细菌细胞的特异性寄生生物，是抗生素替代品之一，可以有助于食品安全和保障。本研究评估了噬菌体鸡尾酒 SalmoFREE® 在商业肉鸡养殖场控制沙门氏菌的效果。我们评估了使用 SalmoFREE®与生产性能参数（饲料转化率、体重增加、均匀度）之间的关系。在哥伦比亚一家有沙门氏菌存在记录的肉鸡养殖场的商业饲养条件下进行了两项现场试验（试验 1 n = 34986；试验 2 n = 34680）。每个试验包括 2 个对照鸡舍和 2 个试验鸡舍。SalmoFREE®和对照悬浮液在生产周期的 3 个时间点添加到饮用水中，并在处理前和处理后一天评估直肠拭子中沙门氏菌的存在情况。结果表明，SalmoFREE®可控制沙门氏菌的发病率，且不会影响动物或生产参数，证明其在生产规模上的有效性和无害性。我们在使用 SalmoFREE®处理后从盲肠中提取的总 DNA 样本中检测到噬菌体特异性基因，并测试了鸡尾酒耐药沙门氏菌的出现情况，结果表明这是一种罕见的事件。这些结果为在肉鸡养殖场采用噬菌体疗法作为抗生素促生长替代品提供了相关信息。
