Kucharska-Pulczynska M, Ellegaard J, Hokland P
Br J Haematol. 1987 Apr;65(4):395-9. doi: 10.1111/j.1365-2141.1987.tb04139.x.
Peripheral blood and bone marrow mononuclear cells from 10 patients with newly diagnosed Waldenström's macroglobulinaemia were analysed for the presence of leucocyte differentiation antigens using flow cytometry. Most patients had circulating intracytoplasmic-mu cells that were positive for the B-cell specific antigens B1, B2 and B4 and for the plasma cell antigen PCA-1. When such monoclonal cyto-mu + cells were purified by immune rosette depletion they were shown to express B1 to a much lesser extent than B2, B4 and PCA-1. In bone marrow samples cells with a similar phenotype, but with higher B1 expression were found in all patients. When the serum IgM paraprotein concentrations were compared to surface markers longitudinally from presentation and during the first courses of chemotherapy, a decreased serum IgM level was accompanied by a decrease in B1 cells in peripheral blood. These data demonstrate that WM cells possess a unique phenotype with coexpression of B-cell and plasma cell associated antigens.
运用流式细胞术分析了10例新诊断的华氏巨球蛋白血症患者的外周血和骨髓单个核细胞中白细胞分化抗原的存在情况。大多数患者循环中的胞质内μ细胞对B细胞特异性抗原B1、B2和B4以及浆细胞抗原PCA-1呈阳性。当通过免疫玫瑰花环去除法纯化此类单克隆胞质μ +细胞时，发现它们表达B1的程度远低于B2、B4和PCA-1。在骨髓样本中，所有患者均发现具有相似表型但B1表达较高的细胞。当纵向比较从初诊时起及化疗第一个疗程期间的血清IgM副蛋白浓度与表面标志物时，血清IgM水平降低伴随着外周血中B1细胞数量减少。这些数据表明，华氏巨球蛋白血症细胞具有独特的表型，同时表达B细胞和浆细胞相关抗原。
