Martianasari Riesna, Hamid Penny Humaidah
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2 Karangmalang 55281, Yogyakarta, Indonesia.
Vet World. 2019;12(3):367-371. doi: 10.14202/vetworld.2019.367-371. Epub 2019 Mar 4.
is a primary vector of many arthropod-borne diseases. One of the diseases, dengue fever, is an endemic disease in Indonesia causing high mortalities for decades. There are no preventive and specific treatments for dengue so far. Therefore, prevention of this disease largely depends on the mosquito control. Since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. This study aimed to demonstrate the effect of essential oil from L. in the larval and adult stages, as well as its influence on oviposition activity of mosquito.
efficacy was evaluated in various stages of development. For the larvicidal activity, larvae instar III stage was used. Adulticidal assay in this experiment was performed using newly emerged . For oviposition assay, mated was tested for their responses to -treated and non-treated ovitraps.
L. - adulticide activity was effective with a concentration of 2.5 μl/ml, caused 100% mortality within 15-30 min. Larvicide activity was observed after 1 h, 24 h, and 48 h post-treatment with LC183, 92.7, and 59.8 ppm and LC 637, 525, and 434.7 ppm, respectively. Oviposition activity index was -0.917 in 1000 ppm. In addition, the eggs number of oviposition with 100 ppm of essential oil L. was 5 times lower than the control.
This study demonstrated clearly that essential oil derived from L. potentially acts as alternate bioinsecticide to control population. The application can be varied or combined in different stages of mosquito development.
是多种虫媒疾病的主要传播媒介。其中一种疾病——登革热，在印度尼西亚是一种地方病，数十年来导致了很高的死亡率。目前尚无针对登革热的预防和特效治疗方法。因此，这种疾病的预防很大程度上依赖于蚊虫控制。由于全球范围内出现了对化学杀虫剂的抗性，研究替代的新型蚊虫杀虫剂势在必行。本研究旨在证明来自[植物名称未明确]的精油在幼虫和成虫阶段的作用，以及其对[蚊虫名称未明确]产卵活动的影响。
在[蚊虫名称未明确]发育的各个阶段评估其效果。对于杀幼虫活性，使用三龄幼虫阶段。本实验中的成虫杀灭试验使用新羽化的[蚊虫名称未明确]进行。对于产卵试验，测试已交配的[蚊虫名称未明确]对经[精油名称未明确]处理和未处理的诱卵器的反应。
[精油名称未明确]对成虫的杀灭活性在浓度为2.5微升/毫升时有效，在15 - 30分钟内导致100%死亡。用LC183（92.7 ppm和59.8 ppm）和LC 637（525 ppm和434.7 ppm）处理后1小时、24小时和48小时观察到杀幼虫活性。在1000 ppm时产卵活性指数为 -0.917。此外，用100 ppm的[精油名称未明确]精油处理后，[蚊虫名称未明确]的产卵数量比对照低5倍。
本研究清楚地表明，来自[植物名称未明确]的精油有潜力作为替代生物杀虫剂来控制[蚊虫名称未明确]种群。其应用可以在蚊虫发育的不同阶段进行变化或组合。
